Blocking effect of lyt-2 antibodies on T cell functions
Blocking effect of lyt-2 antibodies on T cell functions
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lyt-2 抗体对 T 细胞功能的阻断作用
DOI：
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发表时间：
1980
影响因子：
15.3
通讯作者：
Irving L. Weissman
中科院分区：
文献类型：
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作者：
Irving L. Weissman
单克隆抗Lyt-2抗体在不存在添加的补体的情况下阻断细胞毒性胸腺衍生（T）细胞的效应子功能。在效应淋巴细胞水平抑制同种异体细胞和同系淋巴瘤或肉瘤靶细胞的细胞溶解。抗Lyt-1和抗Thy-1抗体不阻断杀伤细胞。混合淋巴细胞培养物中T细胞的增殖也被抗Lyt-2抑制，但不受抗Lyt-1或抗Thy-1抗体的影响。尽管混合淋巴细胞反应中需要Lyt-1+淋巴细胞作为Lyt-2+淋巴细胞增殖的辅助细胞，但它们的辅助功能不受Lyt-1抗体存在的影响。因此，尽管抗Lyt-1、抗Lyt-2和抗Thy-1属于相同的γ 2A免疫球蛋白类别，具有高滴度，并且以相同的程度与T细胞相互作用，但只有抗Lyt-2阻断T细胞功能。与同种异体抗原的激活相反，由伴刀豆球蛋白A对T淋巴细胞的多克隆激活不被Lyt-2抗体抑制，这表明Lyt-2抗体在T细胞抗原受体水平上干扰T细胞功能。Lyt-2分子可能在T细胞功能中发挥的作用进行了讨论。
Monoclonal anti-Lyt-2 antibodies blocked effector function of cytotoxic thymus-derived (T) cells in the absence of added complement. Cytolysis of both allogeneic cells and syngeneic lymphoma or sarcoma target cells was inhibited at the level of the effector lymphocytes. Anti-Lyt-1 and anti-Thy-1 antibodies did not block killer cells. Proliferation of T cells in mixed lymphocyte culture was also inhibited by anti-Lyt-2, but not affected by anti-Lyt-1 or anti-Thy-1 antibodies. Although Lyt-1+ lymphocytes were required in the mixed lymphocyte reaction as helper cells for proliferation of Lyt-2+ lymphocytes, their helper function was not affected by the presence of Lyt-1 antibodies. Thus, although anti-Lyt-1, anti-Lyt-2 and anti-Thy-1 were of the same gamma 2A immunoglobulin class, had high titers, and interacted with T cells to the same extent, only anti-Lyt-2 blocked T cell functions. Polyclonal activation of T lymphocytes by concanavalin A, in contrast to activation by alloantigens, was not inhibited by Lyt-2 antibodies, suggesting that Lyt-2 antibodies interfere with T cell function at the level of the T cell antigen-receptor. The role which Lyt-2 molecules may play in T cell function is discussed.