Transcriptome analysis revealed novel possible venom components and cellular processes of the tarantula Chilobrachys jingzhao venom gland
Transcriptome analysis revealed novel possible venom components and cellular processes of the tarantula Chilobrachys jingzhao venom gland
复制标题
转录组分析揭示了狼蛛 Chilobrachys jingzhao 毒腺的新的可能毒液成分和细胞过程。
DOI：
10.1016/j.toxicon.2008.08.003
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发表时间：
2008-12-01
期刊：
影响因子：
2.8
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Chen, Jinjun;Zhao, Liqun;Liang, Songping
京兆狼蛛（Tarantula Chilobrachys jingzhao）是一种毒性最强的蜘蛛，具有合成和分泌复杂而丰富的毒蛋白的专门器官--毒腺。毒液的成分已被广泛研究。目前对毒素分泌和代谢的分子机制仍知之甚少。为了更全面地了解其毒腺的功能，我们构建了一个非标准化的毒腺cDNA文库，并获得了788个表达序列标签（EST）。所有EST序列被组装成356个非冗余序列，包括85个簇和271个单联体，其中31.4%的独特序列属于分泌蛋白编码序列，包括胱氨酸结毒素（29.1%）和其他分泌蛋白（2.3%），54.0%与常见的细胞转录本相似，14.6%与任何已知序列无显著相似性。这些EST的注释揭示了一些新的可能的毒液成分和细胞过程的重要毒腺功能，包括蛋白质翻译后加工，细胞运动，蛋白质合成，能量供应等。这项研究包含了一个转录组分析蜘蛛毒腺专注于其细胞结构和功能方面，并确认了非常专业的性质蜘蛛毒腺毒素生产商。(C)2008爱思唯尔有限公司保留所有权利。
The tarantula Chilobrachys jingzhao is one of the most venomous spiders with a specialized organ, venom gland, which synthesizes and secretes the complex and abundant toxin proteins. The components of the venom have been extensively studied. As far as the molecular mechanism of toxin secretion and metabolism is concerned, we still knew a little. To obtain a comprehensive view of function of its venom gland we constructed a non-normalized cDNA library of the venom gland and generated 788 expressed sequence tags (ESTs). All ESTs were assembled into 356 non-redundancy sequences including 85 clusters and 271 singlets, of which 31.4% of total unique sequences belong to secretion protein coding sequences including cystine knot toxins (29.1%) and other secretion Proteins (2.3%); 54.0% are similar to common cellular transcripts: and 14.6% have no significant similarity to any known sequences. Annotation of these ESTs revealed some novel possible venom components and cellular processes important for venom gland functions, including protein posttranslation processing, cell motility, protein synthesis, energy supply, etc. This study contains a transcriptome analysis of spider venom gland focusing on its cellular structural and functional aspects, and confirms the very specialized nature of the spider venom gland as toxin producer. (C) 2008 Elsevier Ltd. All rights reserved.