Essential oil of Melaleuca alternifolia for the treatment of oral candidiasis induced in an immunosuppressed mouse model.
Essential oil of Melaleuca alternifolia for the treatment of oral candidiasis induced in an immunosuppressed mouse model.
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DOI：
10.1186/1472-6882-14-489
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发表时间：
2014-12-15
影响因子：
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通讯作者：
Junqueira, Juliana Campos
中科院分区：
文献类型：
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作者：
de Campos Rasteiro, Vanessa Maria;da Costa, Anna Carolina Borges Pereira;Junqueira, Juliana Campos
背景技术背景：寻找口腔念珠菌病的替代疗法是必要的，使用药用植物似乎是有前途的解决方案之一。目的：研究互叶白千层挥发油对白色念珠菌的体外和体内抑菌作用。通过肉汤微量稀释测定来确定互叶米草。对于体内研究，12只患有口腔念珠菌病的免疫抑制小鼠接受局部应用M。alternifolia与MBEC。处理后，从小鼠中回收酵母并定量（CFU/mL）。处死小鼠，通过光学和扫描电子显微镜对舌背进行形态学分析。结果：M.互花米草的MBEC为0.195%，MBEC为12.5%。治疗M。alternifolia对C.结论：M. 12.5%的互叶草油可有效地杀灭黑胫病。白念珠菌生物被膜的形成，并减少念珠菌的酵母菌。白色念珠菌在免疫抑制小鼠模型。
BACKGROUND: The search for alternative therapies for oral candidiasis is a necessity and the use of medicinal plants seems to be one of the promising solutions. The objective of this study was to evaluate the in vitro and in vivo effects of the essential oil of Melaleuca alternifolia on Candida albicans.METHODS: The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of M. alternifolia were determined by the broth microdilution assay. For the in vivo study, twelve immunosuppressed mice with buccal candidiasis received topical applications of M. alternifolia with MBEC. After treatment, yeasts were recovered from the mice and quantified (CFU/mL). Mice were killed for morphologic analysis of the tongue dorsum by optical and scanning electron microscopy. Data were analyzed using Student's t test or Mann-Whitney test.RESULTS: The MIC of M. alternifolia was 0.195% and the MBEC was 12.5%. Treatment with M. alternifolia achieved a 5.33 log reduction in C. albicans and reduced the microscopic lesions of candidiasis.CONCLUSIONS: M. alternifolia oil at a 12.5% was effective to eradicate a C. albicans biofilm formed in vitro and to reduce yeasts of C. albicans in an immunosuppressed mouse model.