THE CHEMOKINE, EOTAXIN, ACTIVATES GUINEA-PIG EOSINOPHILS IN-VITRO AND CAUSES THEIR ACCUMULATION INTO THE LUNG IN-VIVO
THE CHEMOKINE, EOTAXIN, ACTIVATES GUINEA-PIG EOSINOPHILS IN-VITRO AND CAUSES THEIR ACCUMULATION INTO THE LUNG IN-VIVO
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DOI：
10.1006/bbrc.1993.2599
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发表时间：
1993-12-30
影响因子：
3.1
通讯作者：
WILLIAMS, TJ
中科院分区：
文献类型：
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作者：
GRIFFITHSJOHNSON, DA;COLLINS, PD;WILLIAMS, TJ
嗜酸粒细胞趋化因子是一种新型的C-C趋化因子，从气溶胶过敏原激发后主动致敏豚鼠的支气管肺泡灌洗液（BAL）中纯化。在这项研究中，我们表明，eotaxin诱导豚鼠嗜酸性粒细胞在体外细胞内游离钙浓度（[Ca 2 +]i）和聚集的剂量依赖性增加。皮内注射嗜酸性粒细胞活化趋化因子诱导[111 In]嗜酸性粒细胞在豚鼠皮肤中的蓄积，但无水肿诱导活性;后者出现在单独的BAL液HPLC组分中。气溶胶暴露的幼稚豚鼠体内嗜酸性粒细胞趋化因子引起的选择性增加，嗜酸性粒细胞在BAL液。因此，嗜酸性粒细胞趋化因子在体外激活豚鼠嗜酸性粒细胞，并导致嗜酸性粒细胞在体内肺内的选择性积聚。嗜酸性粒细胞趋化因子及其相关分子是体内过敏反应中潜在的重要内源性信号物质。
Eotaxin is a novel C-C chemokine purified from the bronchoalveolar lavage (BAL) fluid of actively sensitised guinea-pigs after aerosol allergen challenge. In this study we show that eotaxin induced a dose-dependent increase in both intracellular-free calcium concentration ([Ca2+]i) and aggregation of guinea-pig eosinophilsin vitro. Intradermally injected eotaxin induced the accumulation of [111In]eosinophils in naive guinea-pig skinin vivo, without oedema-inducing activity; the latter emerging in separate BAL fluid HPLC fractions. Aerosol exposure of naive guinea-pigs to eotaxinin vivocaused a selective increase in eosinophils in BAL fluid. Thus, eotaxin activates guinea-pig eosinophilsin vitroand causes a selective eosinophil accumulation in the lungin vivo. Eotaxin and related molecules are potentially important endogenous signalling substances in allergic reactionsin vivo.