Grape seed proanthocyanidins improved cardiac recovery during reperfusion after ischemia in isolated rat hearts
Grape seed proanthocyanidins improved cardiac recovery during reperfusion after ischemia in isolated rat hearts
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DOI：
10.1093/ajcn/75.5.894
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发表时间：
2002-05-01
影响因子：
7.1
通讯作者：
Tosaki, A
中科院分区：
文献类型：
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作者：
Pataki, T;Bak, I;Tosaki, A
背景：越来越多的证据表明，饮用红葡萄酒具有心脏保护作用。这些影响已被归因于多酚类化合物在grapes.Objective：我们研究了红葡萄籽原花青素对恢复缺血后功能的影响在离体大鼠hearts.Design：两组大鼠喂养不同剂量的原花青素丰富的提取物3周，另一组是未经处理的，作为对照。然后将动物麻醉，分离心脏并进行30分钟的缺血，然后再灌注2小时。冠状动脉流出物收集在第三分钟的再灌注测量氧自由基通过使用电子自旋共振spectroscopic.Results：在大鼠治疗与50和100毫克葡萄籽原花青素/kg，再灌注诱导的心室颤动的发病率从其控制值的92%降低到42%和25%。两组比较P < 0.05。室性心动过速的发生率也呈现相同的规律。给予100 mg/kg原花青素的大鼠，冠脉流量、主动脉流量恢复。再灌注60 min后的血压和发展压分别比未处理对照组提高32% ± 8%、98% ± 8%和37% ± 3%（均P < 0.05）。电子自旋共振研究表明，原花青素显着抑制氧自由基的形成。100 mg/kg葡萄籽原花青素组大鼠自由基浓度较对照组降低75% ± 7%（P < 0.05）。结论：葡萄籽原花青素通过直接或间接地减少或清除缺血再灌注心肌中的自由基，对缺血再灌注损伤具有心肌保护作用。
Background: Increasing evidence shows that red wine consumption has cardioprotective effects. These effects have been attributed to the polyphenolic compounds in grapes.Objective: We studied the effects of red grape seed proanthocyanidins on the recovery of postischemic function in isolated rat hearts.Design: Two groups of rats were fed different doses of proanthocyanidin-rich extract for 3 wk and another group was untreated and served as controls. The animals were then anesthetized and the hearts were isolated and subjected to 30 min of ischemia followed by 2 h of reperfusion. Coronary effluents were collected during the third minute of reperfusion for measurement of oxygen free radicals by using electron spin resonance spectroscopy.Results: In rats treated with 50 and 100 mg grape seed proanthocyanidins/kg, the incidence of reperfusion-induced ventricular fibrillation was reduced from its control value of 92% to 42% and 25%. respectively (P < 0.05 for both). The incidence of ventricular tachycardia showed the same pattern. In rats treated with 100 mg proanthocyanidins/kg, the recovery of coronary flow, aortic flow. and developed pressure after 60 min of reperfusion was improved by 32% +/- 8%, 98% +/- 8%, and 37% +/- 3%, respectively (P < 0.05 for all) compared with untreated control rats. Electron spin resonance studies indicated that proanthocyanidins significantly inhibited the formation of oxygen free radicals. In rats treated with 100 mg proanthocyanidins/kg, free radical intensity was reduced by 75% +/- 7% (P < 0.05) compared with the control rats.Conclusion: Grape seed proanthocyanidins have cardioprotective effects against reperfusion-induced injury via their ability to reduce or remove, directly or indirectly, free radicals in myocardium that is reperfused after ischemia.