QUANTITATIVE ASSESSMENT OF HUMAN PROTEINASES AS AGENTS FOR CHEMONUCLEOLYSIS
QUANTITATIVE ASSESSMENT OF HUMAN PROTEINASES AS AGENTS FOR CHEMONUCLEOLYSIS
复制标题
DOI：
10.1097/00007632-198802000-00010
复制
发表时间：
1988-02-01
期刊：
影响因子：
3
通讯作者：
BARRETT, AJ
中科院分区：
文献类型：
--
作者：
DANDO, PM;MORTON, DB;BARRETT, AJ
描述了一种兔模型系统。它可以准确地测量注射蛋白酶后腰椎间盘髓核中糖胺多糖的剂量依赖性损失。在相当于用于人类化学核溶解的木瓜凝乳酶的剂量下，两种人类丝氨酸蛋白酶，组织蛋白酶G和凝乳酶，在从椎间盘中去除高达80%的糖胺多糖方面与木瓜凝乳酶一样有效。组织蛋白酶B是一种半胱氨酸蛋白酶，其释放的糖胺聚糖不超过45%。当去除30-40%的糖胺多聚糖时，X光片清楚地显示椎间盘间隙变窄。髓核的降解在组织学上表现为甲苯胺蓝异染色质的消失。
A rabbit model system is described. It allows accurate measurement of the dose-dependent loss of glycosaminoglycan from the nucleus pulposus of lumbar intervertebral discs after injection of a proteinase. At the dose equivalent to that of chymopapain used in human chemonucleolysis, two human serine proteinases, cathepsin G and chymotrypsin, were as effective as chymopapain in removing up to 80% of the glycosaminoglycan from the disc. A cysteine proteinase, cathepsin B released no more than 45% of glycosaminoglycan. X-ray films clearly showed narrowing of the disc space when 30-40% of glycosaminoglycan was removed. The degradation of the nucleus pulposus was seen histologically as loss of toluidine blue metachromasia.