The ferret: a new model of oral ethanol injury involving the liver, bone marrow, and peripheral blood lymphocytes.
The ferret: a new model of oral ethanol injury involving the liver, bone marrow, and peripheral blood lymphocytes.
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雪貂：一种涉及肝脏、骨髓和外周血淋巴细胞的口服乙醇损伤的新模型。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
A. Chedid
中科院分区：
文献类型：
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作者：
G. Roselle;C. Mendenhall;A. Muhleman;A. Chedid
我们已经开发了一个模型，通过提供一个完整的液体饮食，其中21%的总热量摄入是乙醇在雪貂口服乙醇摄入。乙醇处理3周后，雪貂淋巴细胞的迁移抑制因子活性与用葡萄糖替代的相同液体饲料相比显著降低。在摄入乙醇6周后，淋巴细胞对有丝分裂原植物血凝素的反应也减少了。治疗11周后，组织病理学上发现肝细胞变性、脂肪沉积和“Mallory体样”物质。摄入乙醇6周后，小鼠髂嵴骨髓抽吸细胞红细胞和髓细胞均出现空泡化。乙醇对照组和葡萄糖对照组雪貂平均体重差异不显著；在研究期间，与人类酗酒者相比，血清乙醇和乙醛浓度略高。因此，我们设计了一个酒精毒性作用的小动物模型，其中多个器官系统显示出酒精相关疾病的证据。
We have developed a model of oral ethanol ingestion in the ferret by providing a complete liquid diet in which 21% of the total caloric intake is given as ethanol. After 3 weeks of ethanol treatment, migration inhibitory factor activity from ferret lymphocytes was significantly decreased when compared to animals fed a dextrose-substituted, identical liquid diet. Lymphocyte blastogenesis in response to the mitogen, phytohemagglutinin, was also decreased after 6 weeks of ethanol ingestion. Histopathologically, hepatic cell degeneration, fat deposition, and "Mallory body-like" material were present after 11 weeks of therapy. Bone marrow aspirate cells from the iliac crest of ethanol-fed animals showed vacuolization in both erythroid and myeloid elements after 6 weeks of ethanol ingestion. Mean ferret weights were not significantly different between the ethanol and dextrose control groups; serum ethanol and acetaldehyde concentrations were somewhat higher over the study period compared to those seen in human alcoholics. Therefore, we have designed a small animal model of the toxic effects of alcohol in which multiple organ systems exhibited evidence of ethanol-related disease.