Patterns of MUC1 tissue expression defined by an anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
Patterns of MUC1 tissue expression defined by an anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer
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DOI：
10.1177/002215540305100609
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发表时间：
2003-06-01
影响因子：
3.2
通讯作者：
Segal-Eiras, A
中科院分区：
文献类型：
--
作者：
Croce, MV;Isla-Larrain, MT;Segal-Eiras, A
我们的目的是确定模式的表达MUC 1粘蛋白胞浆尾（MUC 1 CT）在乳腺癌。共98例浸润性乳腺癌肿瘤样本进行了免疫组化（IHC）分析。反应模式分为膜型、胞质型或混合型。在SDS-PAGE和Western印迹后制备亚细胞级分。使用的抗体是抗MUC 1 CT（CT 2单克隆抗体，MAb）和抗细胞外MUC 1核心蛋白的C595 MAb。使用CT 2 MAb，IHC显示93%的标本中阳性染色百分比较高，膜染色是观察到的最常见模式。C595单克隆抗体在73%的标本中呈反应性。膜和细胞质染色的百分比相似，主要是在混合模式。Western blotting显示不同的条带。使用CT 2 MAb时，膜部分显示出最强烈的反应;在约< 30 kD处检测到强反应条带。用C595单克隆抗体，在大多数情况下，在200 kD的双带被发现。在乳腺上皮中，MUC 1 CT表达的模式可能构成MUC 1生产的指标，因为它不依赖于糖基化。MUC 1 CT阳性的模式和范围不随肿瘤的组织病理学亚型而变化。
Our aim was to determine the pattern of expression of MUC1 mucin cytoplasmic tail (MUC1 CT) in breast carcinoma. A total of 98 invasive breast adenocarcinoma tumor samples were assayed by immunohistochemical (IHC) analysis. The pattern of reaction was classified as membrane, cytoplasmic, or mixed. Subcellular fractions were prepared after SDS-PAGE and Western blotting. The antibodies employed were anti-MUC1 CT (CT2 monoclonal antibody, MAb) and C595 MAb against the extracellular MUC1 core protein. With the CT2 MAb, IHC showed a high percentage of positive staining in 93% of specimens, with membrane staining the most common pattern observed. C595 MAb was reactive in 73% of specimens. Similar percentages of membrane and cytoplasmic staining were found, mainly in a mixed pattern. Western blotting showed different bands. With the CT2 MAb, the membrane fraction showed the most intense reaction; a strong band of reaction was detected at approximately < 30 kD. With the C595 MAb, in most cases a double band at 200 kD was found. In breast epithelium, the pattern of MUC1 CT expression may constitute an indicator of MUC1 production because it does not depend on glycosylation. The pattern and extension of MUC1 CT positivity do not vary according to the histopathological subtype of the tumor.