Berberine attenuates depressive-like behaviors by suppressing neuro-inflammation in stressed mice
Berberine attenuates depressive-like behaviors by suppressing neuro-inflammation in stressed mice
复制标题
小檗碱通过抑制应激小鼠的神经炎症来减轻抑郁样行为
DOI：
10.1016/j.brainresbull.2017.08.008
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发表时间：
2017-09-01
影响因子：
3.8
通讯作者：
Yi, Li-Tao
中科院分区：
文献类型：
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作者：
Liu, Ya-Min;Niu, Le;Yi, Li-Tao
小檗碱是从中药黄连中提取的主要生物碱成分，在啮齿类动物中具有抗抑郁作用。然而，小檗碱的作用是否涉及神经炎症抑制尚不清楚。本研究旨在探讨小檗碱对慢性轻度不可预知应激（CHMS）小鼠神经炎症系统的影响。小檗碱连续4周灌胃给药。行为学评价结果表明，小檗碱对蔗糖偏好试验和新奇抑制摄食试验中的抑郁障碍均有预防作用。小檗碱降低海马促炎细胞因子如白细胞介素-1 β（IL-1 β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的升高以及小胶质细胞的活化。此外，慢性小檗碱治疗抑制了活化B细胞核因子κ轻链增强子（NF-κ B）信号传导途径，因为小檗碱给药后海马中NF-κ B、I κ B激酶（IKK）α和IKK β的磷酸化蛋白受到抑制。此外，小檗碱还抑制NF-κ B B信号通路的下游靶点诱导型一氧化氮合酶（iNOS）。总之，这些研究结果表明，黄连素的管理可以防止抑郁样行为在BARS小鼠通过抑制海马神经炎症。
Berberine, the major constituent alkaloid originally from the famous Chinese herb Huanglian (Coptis chinensis), has been shown to exert antidepressant-like effects in rodents. However, it is still not clear the involvement of neuro-inflammation suppression in the effects of berberine. The purpose of this study was to determine whether berberine affects the neuro-inflammation system in mice induced by chronic unpredictable mild stress (CUMS). Berberine was orally administrated in normal or CUMS mice for successive four weeks. Behavioral evaluation showed that berberine prevented the depressive deficits both in sucrose preference test and novelty-suppressed feeding test. The elevation of hippocampal pro-inflammatory cytokines such as interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), as well as the activation of microglia were decreased by berberine. In addition, chronic berberine treatment inhibited nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) signaling pathway as the phosphorylated proteins of NF-kappa B, I kappa B kinase (IKK)alpha and IKK beta in the hippocampus were suppressed after berberine administration. Furthermore, inducible nitric oxide synthase (iNOS), one downstream target of NF-kappa B signaling pathway was also inhibited by berberine. In conclusion, these findings suggest that administration of berberine could prevent depressive-like behaviors in CUMS mice by suppressing neuro-inflammation in the hippocampus.