Extracellular pyridine nucleotides trigger plant systemic immunity through a lectin receptor kinase/BAK1 complex
Extracellular pyridine nucleotides trigger plant systemic immunity through a lectin receptor kinase/BAK1 complex
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DOI：
10.1038/s41467-019-12781-7
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发表时间：
2019-10-22
影响因子：
16.6
通讯作者：
Mou, Zhonglin
中科院分区：
文献类型：
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作者：
Wang, Chenggang;Huang, Xiaoen;Mou, Zhonglin
系统获得性抗性（SAR）是一种持久的广谱植物免疫，由最初感染发生的局部叶子产生的移动信号诱导。尽管已经提出了多种结构上不相关的信号，但在系统叶中感知这些信号的机制尚不清楚。在这里，我们证明外源性应用的烟酰胺腺嘌呤二核苷酸（NAD（+））可以全身移动并诱导全身免疫。我们证明，凝集素受体激酶 (LecRK) LecRK-VI.2 是细胞外 NAD(+) (eNAD(+)) 和 NAD(+) 磷酸盐 (eNADP(+)) 的潜在受体，并在 SAR 的生物诱导中发挥核心作用。 LecRK-VI.2 在体内与油菜素类固醇不敏感 1 相关激酶 1 (BAK1) 组成型关联。此外，BAK1 及其同源物 BAK1-LIKE1 是 eNAD(P)(+) 信号传导和 SAR 所必需的，并且 LecR-VI.2 和 BAK1 的激酶活性对于它们在 SAR 中的功能是不可或缺的。我们的结果表明，eNAD(+) 是一种假定的移动信号，它通过拟南芥中的受体复合物 LecRK-VI.2/BAK1 触发 SAR。
Systemic acquired resistance (SAR) is a long-lasting broad-spectrum plant immunity induced by mobile signals produced in the local leaves where the initial infection occurs. Although multiple structurally unrelated signals have been proposed, the mechanisms responsible for perception of these signals in the systemic leaves are unknown. Here, we show that exogenously applied nicotinamide adenine dinucleotide (NAD(+)) moves systemically and induces systemic immunity. We demonstrate that the lectin receptor kinase (LecRK), LecRK-VI.2, is a potential receptor for extracellular NAD(+) (eNAD(+)) and NAD(+) phosphate (eNADP(+)) and plays a central role in biological induction of SAR. LecRK-VI.2 constitutively associates with BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) in vivo. Furthermore, BAK1 and its homolog BAK1-LIKE1 are required for eNAD(P)(+) signaling and SAR, and the kinase activities of LecR-VI.2 and BAK1 are indispensable to their function in SAR. Our results indicate that eNAD(+) is a putative mobile signal, which triggers SAR through its receptor complex LecRK-VI.2/BAK1 in Arabidopsis thaliana.