Bone tissue engineering electrospun scaffolds based on layered double hydroxides with the ability to release vitamin D3: Fabrication, characterization and in vitro study
Bone tissue engineering electrospun scaffolds based on layered double hydroxides with the ability to release vitamin D3: Fabrication, characterization and in vitro study
复制标题
DOI：
10.1016/j.clay.2019.105434
复制
发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Hashjin, Mehran Solati
中科院分区：
文献类型：
--
作者：
Belgheisi, Ghazal;Nazarpak, Masoumeh Haghbin;Hashjin, Mehran Solati
骨组织工程支架可以促进和加速组织再生，并控制药物和信号分子的释放，同时为细胞粘附和增殖提供合适的条件。目前的研究涉及制造聚（ε-己内酯）（PCL）电纺支架富含维生素D3（VD 3）负载的层状双氢氧化物（LDHs）纳米杂化物，在不同的浓度。表征证实了LDH的成功合成和晶面的形成以及VD 3在LDH中的嵌入，包封率为65%。添加低浓度的LDHs纳米杂化材料到PCL支架导致纤维直径的减小;然而，在较高浓度下观察到表面粗糙度的增加。还评估了支架的机械性能和孔隙率，结果显示纳米杂化物富集支架的伸长率模量降低，并且未观察到孔隙率百分比的显著差异。模拟体液（10 X SBF）中支架的生物活性研究表明，VD 3促进了体外磷灰石样晶体的形成。此外，纳米杂化材料的加入增加了支架的降解速率。VD 3的累积释放显示出相同的模式，所有支架通过扩散作为主要的释放控制机制。利用MG-63细胞系培养研究支架的体外生物学反应。结果表明，含纳米杂化材料的支架可以高度支持细胞粘附和增殖。同样，碱性磷酸酶活性作为支架的骨传导性的指标，在含有纳米杂化物的PCL支架和纯PCL支架之间没有显着差异（p > 0.05）。结果证实了负载VD 3的LDH/PCL电纺支架用于骨组织工程的潜力。
Bone tissue engineered scaffolds can improve and accelerate the tissue regeneration alongside controlled release of drugs and signaling molecules while providing a suitable condition for cell adhesion and, proliferation. The current study involves the fabrication of Poly (epsilon-caprolactone) (PCL) electrospun scaffolds enriched with vitamin D3 (VD3)-loaded layered double hydroxides (LDHs) nanohybrid, in different concentrations. Characterizations confirmed successful synthesis of LDH and formation of crystalline planes and intercalation of VD3 in LDH with 65% encapsulation efficiency. Addition of low concentrations of LDHs nanohybrid to PCL scaffold led to a decrease in the fiber diameter; however, an increase in the surface roughness was observed at higher concentrations. Mechanical properties and porosity of the scaffolds were also evaluated and the results showed a decrease in elongation modulus in nanohybrid enriched scaffolds and no significant differences in porosity percentage was observed. Studies on bioactivity of scaffolds subjected to simulated body fluid (10 X SBF) indicated that the VD3 encouraged the formation of apatite-like crystals in vitro. Furthermore, the degradation rate of scaffolds increased due to the addition of nanohybrid. The cumulative release of VD3 showed the same pattern for all scaffolds through the diffusion as the main controlling mechanism of release. In vitro biological responses of the scaffolds were studied using MG-63 cell lines culture. The results indicated that nanohybrid containing scaffolds could highly support cell adhesion and proliferation. Likewise, alkaline phosphatase activity as an indicator of osteoconductivity for scaffolds showed no significant differences between nanohybrid containing and pure PCL scaffolds (p > .05). The results confirmed the potential of VD3-loaded LDH/PCL electrospun scaffolds for bone tissue engineering.