Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases.
Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases.
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克隆大鼠肾脏 cDNA，该 cDNA 可刺激二元和中性氨基酸转运，并与葡萄糖苷酶具有序列相似性。
DOI：
10.1073/pnas.89.12.5596
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发表时间：
1992
影响因子：
11.1
通讯作者：
Hediger,MA
中科院分区：
文献类型：
--
作者：
Wells,RG;Hediger,MA
氨基酸的跨膜转运被认为是由具有不同底物特异性的膜整合蛋白介导的。利用爪蟾卵母细胞的表达克隆和14 C-标记胱氨酸的摄取测定，我们从大鼠肾脏文库中分离出2.3-脱氢酶cDNA（D2）。D2在肾脏和肠道中特异性表达，并诱导中性和阳离子氨基酸的转运。推导的氨基酸序列预测一个78 kDa的蛋白质与一个单一的跨膜结构域，一个结构不典型的已知的膜转运蛋白，通常有多个跨膜区域。推定的细胞外区域是高度相似的4F 2重链细胞表面抗原和α-葡糖苷酶家族，这提高了D2编码转运激活剂或调节亚基的可能性。
The transport of amino acids across cell membranes is believed to be mediated by integral membrane proteins with distinct substrate specificities. Using expression cloning in Xenopus oocytes and assaying for the uptake of 14C-labeled cystine, we isolated a 2.3-kilobase cDNA (D2) from a rat kidney library. D2 is expressed specifically in kidney and intestine and induces the transport of both neutral and cationic amino acids. The deduced amino acid sequence predicts a 78-kDa protein with a single transmembrane domain, a structure not typical of the known membrane transport proteins, which generally have multiple membrane-spanning regions. The putative extracellular region is highly similar to the 4F2 heavy-chain cell surface antigen and to a family of alpha-glucosidases, which raises the possibility that D2 encodes a transport activator or regulatory subunit.