Evaluation of inflammatory responses against muscle larvae of different Trichinella species by an image analysis system
Evaluation of inflammatory responses against muscle larvae of different Trichinella species by an image analysis system
复制标题
DOI：
10.1016/j.vetpar.2008.10.038
复制
发表时间：
2009-02-23
影响因子：
2.6
通讯作者：
Masetti, M.
中科院分区：
文献类型：
--
作者：
Bruschi, F.;Marucci, G.;Masetti, M.
本研究的目的是评价肌肉组织对旋毛虫幼虫包囊（T。spiralis、T. britovi）和非包囊（T pseudospiralis）物种。通过一种新开发的方法，在旋毛虫感染的CD 1小鼠的肌肉组织的组织切片中估计炎症反应。用一种以高亲和力结合核酸的荧光探针对核染色，并通过软件程序分析荧光。在囊周（靠近滋养细胞-寄生虫复合体）和囊外（寄生虫不可见）区域进行相对荧光单位评价。旋毛虫感染的小鼠肌肉组织中细胞核数量的增加被认为是炎症标志物，因为未感染的肌肉显示低细胞核密度。为了评价微囊化的旋毛虫、旋毛虫和旋毛虫之间亚硝基化模式的差异，britovi）和未包封的（T. pseudospiralis、Trichinellapapapuae、Trichinellazimbabwensis）种，L-1幼虫通过用抗硝基酪氨酸多克隆抗体的免疫印迹进行测试。旋毛虫幼虫在肌肉组织中引起的炎症在统计学上高于其他物种引起的，无论是在囊外和囊外区域。包封物种的亚硝基化水平高于非包封物种。在这项工作中开发的方法可以证明宿主对包囊和非包囊旋毛虫物种的炎症反应的差异。(C)2008 Elsevier B. V.保留所有权利。
The aim of this study was to evaluate the inflammatory response in the muscle tissue against Trichinella larvae of encapsulated (T. spiralis, T. britovi) and non-encapsulated (T pseudospiralis) species. The inflammatory response was estimated in histological sections of muscle tissues from Trichinella-infected CD1 mice by a newly developed method. Nuclei were stained with one fluorescent probe, which binds nucleic acids with high affinity, and fluorescence was analysed by a software program. Evaluation of the relative fluorescence units was performed in both peri-capsular (close to the nurse cell-parasite complex) and extra-capsular (where the parasite was not visible) areas. The increase in the number of nuclei in the muscle tissues of Trichinella-infected mice was considered an inflammation marker, since uninfected muscles show low nucleus density. In order to evaluate differences in the nitrosylation pattern between encapsulated (T spiralis, T. britovi) and non-encapsulated (T. pseudospiralis, Trichinella papuae, Trichinella zimbabwensis) species, L-1 larvae were tested by immunoblotting with an anti-nitrotyrosine polyclonal antibody. Inflammation induced by T spiralis larvae in muscle tissues is statistically higher than that elicited by the other species, both in peri- and extra-capsular areas. Nitrosylation occurs at a higher level in encapsulated than in non-encapsulated species. The method developed in this work allows demonstration of differences in the host inflammatory response against encapsulated and non-encapsulated Trichinella species. (C) 2008 Elsevier B.V. All rights reserved.