Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi. IV. Purification and Properties of Plastocyanin.
Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi. IV. Purification and Properties of Plastocyanin.
复制标题
莱茵衣藻的光合电子传递链。
DOI：
10.1104/pp.41.10.1637
复制
发表时间：
1966
期刊：
影响因子：
7.4
通讯作者：
R. Levine
中科院分区：
文献类型：
--
作者：
D. S. Gorman;R. Levine
铜蛋白质质体蓝素已被发现是一个重要组成部分的光合电子传递链的衣原体reinhardi，在本文中，我们描述了一种方法，从野生型菌株的分离和纯化。此外，我们还描述了它的一些性质，并将它们与菠菜质体蓝素的报道进行了比较。从完整细胞制备的丙酮粉末中提取质体蓝素，并通过离子交换层析在DEAE纤维素上和凝胶过滤在Sephadex G-75上进行纯化。纯化的蛋白质的产量范围从相当于2.0至2.5微克原子铜每1000 mumoles叶绿素质体蓝素。一般来说，C. Reinhardi类似于来自菠菜的质体蓝素。与菠菜质体蓝素相比，C. Reinhardi具有更大的吸光度（相对于可见光中的峰）和更少的苯丙氨酸精细结构的证据。C.的正常氧化还原电位。发现Reinhardi质体蓝素为+0.37伏，与菠菜质体蓝素的报道相同。C. Reinhardi质体蓝素估计为13，000 +/-2000。相比之下，菠菜质体蓝素的值已被发现为21，000。
The copper protein plastocyanin has been found to be an essential component of the photosynthetic electron transport chain of Chlamydomonas reinhardi, and in this paper we describe a method for its isolation and purification from the wild-type strain. In addition, we describe some of its properties and compare them with those reported for spinach plastocyanin.The plastocyanin was extracted from acetone powders prepared from intact cells, and it was purified by ion exchange chromatography on DEAE cellulose and gel filtration on Sephadex G-75. The yield of the purified protein ranged from plastocyanin equivalent to 2.0 to 2.5 mug atoms copper per 1000 mumoles chlorophyll. In general the absorption spectrum of plastocyanin from C. reinhardi resembled that of the plastocyanin from spinach. Some spectral differences were found in the ultraviolet region where, in contrast to spinach plastocyanin, that of C. reinhardi had a greater absorbance (relative to peaks in the visible) and less evidence for phenylalanine fine structure. The normal oxidation-reduction potential of C. reinhardi plastocyanin was found to be + 0.37 volts, the same as reported for spinach plastocyanin. The molecular weight of C. reinhardi plastocyanin has been estimated to be 13,000 +/- 2000. In contrast, the value for spinach plastocyanin has been found to be 21,000.