Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes
Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes
复制标题
DOI：
10.1080/22221751.2020.1729071
复制
发表时间：
2020-01-01
影响因子：
13.2
通讯作者：
Zhou, Peng
中科院分区：
文献类型：
--
作者：
Zhang, Wei;Du, Rong-Hui;Zhou, Peng
2019年12月，一种新型冠状病毒(2019-NCoV)在武汉中国引起暴发，并很快蔓延到世界其他地区。人们认为2019-nCoV是通过呼吸道传播并引起肺炎的，因此采用基于口腔拭子的分子诊断来确认该病。同样，患者在口腔拭子中检测到两次阴性后将被释放。然而，许多冠状病毒也可以通过肠道感染通过口腔-粪便途径传播。2019年-nCoV感染患者是否也在肠道等其他器官携带病毒，需要进行检测。我们对当地一家医院感染这种病毒的患者进行了调查。我们在肛拭子和血液中也发现了2019-nCoV，并且在感染的后期，肛拭子阳性的人比口腔拭子阳性的人更多，这表明2019年-nCoV是通过口腔-粪便途径传播的。血清学检测可提高检测阳性率，应在今后的流行病学研究中加以应用。我们的报告发出了警示，2019-nCoV可能会通过多条途径传播。
In December 2019, a novel coronavirus (2019-nCoV) caused an outbreak in Wuhan, China, and soon spread to other parts of the world. It was believed that 2019-nCoV was transmitted through respiratory tract and then induced pneumonia, thus molecular diagnosis based on oral swabs was used for confirmation of this disease. Likewise, patient will be released upon two times of negative detection from oral swabs. However, many coronaviruses can also be transmitted through oral-fecal route by infecting intestines. Whether 2019-nCoV infected patients also carry virus in other organs like intestine need to be tested. We conducted investigation on patients in a local hospital who were infected with this virus. We found the presence of 2019-nCoV in anal swabs and blood as well, and more anal swab positives than oral swab positives in a later stage of infection, suggesting shedding and thereby transmitted through oral-fecal route. We also showed serology test can improve detection positive rate thus should be used in future epidemiology. Our report provides a cautionary warning that 2019-nCoV may be shed through multiple routes.