Serumless Medium for Cultivation of Cells of Normal and Malignant Origin.∗
Serumless Medium for Cultivation of Cells of Normal and Malignant Origin.∗
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用于培养正常和恶性细胞的无血清培养基。*
DOI：
10.3181/00379727-104-25797
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发表时间：
1960
影响因子：
--
通讯作者：
A. Tytell
中科院分区：
文献类型：
--
作者：
R. E. Neuman;A. Tytell
描述了一种适合于Walker癌256、肉瘤180、KB和人心脏细胞体外长时间生长和反复传代培养的无血清培养基。除了氨基酸、维生素、矿物质和葡萄糖外，它还含有胰岛素、硫酸盐碱、丙酮酸、黏液酸、油酸甲酯、亚叶酸和乳酸。除黏液外，后一组材料需要最大的生长速度，与含有透析血清的培养基相当。Walker癌肉瘤256悬浮培养在无血清培养基中连续传代。
Summary A serumless medium suitable for prolonged growth and repeated subculture of Walker carcinosarcoma 256, sarcoma 180, KB and human heart cells in vitro was described. In addition to amino acids, vitamins, minerals and glucose, it contained insulin, salmine sulfate, pyruvate, mucate, methyl oleate, folinic acid and lactalysate. The latter group of materials with exception of mucate was required for maximal growth rates, comparable to those on media containing dialyzed serum. Suspension cultures of Walker carcinosarcoma 256 were grown with continuous passage in the serumless medium.