Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models
Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models
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DOI：
10.1006/dbio.2000.9655
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发表时间：
2000-04-15
影响因子：
2.7
通讯作者：
Brinster, RL
中科院分区：
文献类型：
--
作者：
Shinohara, T;Avarbock, MR;Brinster, RL
精子发生是一个复杂而多产的过程，它起源于精原干细胞，最终导致成熟精子的形成。干细胞在整个生命过程中进行自我更新，但其生物学特性的研究一直很困难，因为睾丸中存在的干细胞数量非常少（每10（4）个细胞中有2至3个），并且只能通过功能来识别。虽然精原细胞移植技术的发展提供了一个干细胞的检测系统，有效的方法来富集干细胞还没有。在这里，我们研究了两种不育小鼠模型，钢/钢（Dickle）（S1/S1（d））和实验隐睾，作为富含干细胞的睾丸细胞群的来源。S1/S1（d）睾丸几乎没有富集，这就提出了关于成体干细胞数量是如何确定的问题，以及目前公认的成体干细胞不依赖于S1因子的观点。与野生型睾丸相比，从隐睾睾丸回收的细胞富集干细胞25倍（集落）或50倍（面积）。隐睾条件不影响干细胞活性，但消除了几乎所有的分化细胞，约1/200的细胞是干细胞。因此，隐睾为精原干细胞的纯化和鉴定提供了一个重要的途径。(C)北京大学出版社.
Spermatogenesis is a complex and productive process that originates from stem cell spermatogonia and ultimately results in formation of mature spermatozoa. The stem cell undergoes self-renewal throughout life, but study of its biological characteristics has been difficult because a very small number (2 to 3 in 10(4) cells) exist in the testis and they can only be identified by function. Although the development of the spermatogonial transplantation technique has provided an assay system for stem cells, efficient methods to enrich stem cells have not been available. Here, we examined two infertile mouse models, Steel/Steel(Dickle)(S1/S1(d)) and experimental cryptorchid, as a source of testis cell populations enriched in stem cells. The S1/S1(d) testis showed little enrichment, which raises questions about how adult stem cell number is determined and about the currently accepted belief that adult stem cells are independent of S1 factor. The cells recovered from cryptorchid testes were enriched for stem cells 25-fold (colonies) or 50-fold (area) compared to wild-type testes. The cryptorchid condition does not affect stem cell activity, but eliminates almost all differentiated cells, and about 1 in 200 cells is a stem cell. Thus, cryptorchid testes provide an important approach for purification and characterization of spermatogonial stem cells. (C) 2000 Academic Press.