ACTIVATION OF ALTERNATE COMPLEMENT PATHWAY BY PEPTIDOGLYCAN FROM STREPTOCOCCAL CELL-WALL
ACTIVATION OF ALTERNATE COMPLEMENT PATHWAY BY PEPTIDOGLYCAN FROM STREPTOCOCCAL CELL-WALL
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DOI：
10.1128/iai.19.1.296-303.1978
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
SCHWAB, JH
中科院分区：
文献类型：
--
作者：
GREENBLATT, J;BOACKLE, RJ;SCHWAB, JH
比较了几种细菌成分对人血清中替代补体途径的激活。按重量计算，A 组链球菌细胞壁的肽聚糖是最活跃的物质，其次是细胞壁、原生质体膜和全细胞。组特异性碳水化合物不活跃。用低浓度的溶菌酶处理肽聚糖或短时间的声波处理可增强补体激活。高浓度的溶菌酶或长时间的声波处理肽聚糖会破坏或大大降低激活补体的能力。溶菌酶处理 A 组链球菌细胞壁或脂多糖没有可测量的效果。 D 组链球菌细胞壁对替代补体途径的激活被溶菌酶破坏。 N-乙酰氨基葡萄糖、N-乙酰胞壁酸或D-丙氨酸-D-丙氨酸不抑制肽聚糖的活性。免疫电泳和交叉免疫电泳显示，肽聚糖可转化 C3 [补体成分 3] 和 B 因子。
Activation of the alternate complement pathway in human serum by several bacterial components was compared. Peptidoglycan from group A streptococcal cell walls was the most active material, on a weight basis, followed by cell walls, protoplast membranes and whole cells. The group-specific carbohydrate was inactive. Treatment of peptidoglycan with low concentrations of lysozyme or short periods of sonic treatment enhanced complement activation. High concentrations of lysozyme or extended sonic treatment of peptidoglycan destroyed or greatly reduced the capacity to activate complement. Lysozyme treatment of group A streptococcal cell walls or lipopolysaccharide had no measurable effect. Activation of the alternate complement pathway by group D streptococcal cell walls was destroyed by lysozyme. Activity of peptidoglycan was not inhibited by N-acetyl glucosamine, N-acetyl muramic acid or D-alanine-D-alanine. Conversion of C3 [complement component 3] and factor B by peptidoglycan was shown to occur by immunoelectrophoresis and crossed immunoelectrophoresis.