Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants
Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants
复制标题
离子束辐照菊花侧芽突变诱导及诱导突变体嵌合结构分析
DOI：
10.1007/s10681-008-9767-5
复制
发表时间：
2008
期刊：
影响因子：
1.9
通讯作者：
T. Morishita
中科院分区：
文献类型：
--
作者：
H. Yamaguchi;Akemi Shimizu;Y. Hase;K. Degi;A. Tanaka;T. Morishita
比较了离子束和γ射线对菊花腋芽的诱变效果，并对诱变株的嵌合结构进行了分析。用2戈伊的碳离子（平均线能量传递122 keV/μm）、10戈伊的氦离子（平均线能量传递9 keV/μm）和80戈伊的γ射线辐照腋芽，对存活率的影响相似。将从辐射芽延伸的芽的下部五个节一个接一个地切割，并且允许新芽从腋芽生长。重复该过程两次，并研究花色突变。通过比较突变体与从根再生的植物的花色来分析嵌合结构。花色突变体的出现频率较高（17.4-28.8%），各处理间突变频率差异不显著。所有的花色突变体都是平周嵌合体。相反，用离子束获得的一些突变体具有与源自根的植物相同的花色。这表明它们是固体突变体，其中LI和LIII组织都来自相同的突变细胞。5戈伊氦离子辐照也获得了固体突变体，其对存活和突变的影响较其他处理小。本文还讨论了仅用离子束获得固体突变体的因素。
We compared the effects of ion beam and gamma ray irradiation on mutation induction in axillary buds of chrysanthemum, and analyzed the chimeric structure of the resulting mutants. Axillary buds were irradiated with carbon ions at 2 Gy (mean linear energy transfer 122 keV/μm), helium ions at 10 Gy (mean 9 keV/μm), and gamma rays at 80 Gy, all of which have the similar effects on survival. The lower five nodes of the shoots elongating from the irradiated buds were cut one by one, and new shoots were allowed to grow from the axillary buds. This procedure was repeated twice, and flower color mutation was investigated. Chimeric structure was analyzed by comparing the flower color of mutants to that of plants regenerated from the roots. Flower color mutants emerged at a high frequency (17.4–28.8%), and there were no significant differences in the mutation frequency between the treatments. All the flower color mutants induced with gamma rays were periclinal chimeras. In contrast, some mutants obtained with ion beams had the same flower color as that of the plants derived from the roots. This suggests that they were solid mutants, where both LI and LIII tissues were derived from the same mutated cell. Solid mutants were also obtained when irradiated with 5 Gy of helium ions, which had less effect on survival and mutation than other treatments. Factors for obtaining solid mutants only with ion beams are also discussed.