CELL LINEAGE-SPECIFIC EXPRESSION OF MODULO, A DOSE-DEPENDENT MODIFIER OF VARIEGATION IN DROSOPHILA
CELL LINEAGE-SPECIFIC EXPRESSION OF MODULO, A DOSE-DEPENDENT MODIFIER OF VARIEGATION IN DROSOPHILA
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DOI：
10.1002/j.1460-2075.1992.tb05548.x
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发表时间：
1992-12-01
期刊：
影响因子：
11.4
通讯作者：
PRADEL, J
中科院分区：
文献类型：
--
作者：
GARZINO, V;PEREIRA, A;PRADEL, J
果蝇的变异是染色质结构在基因表达中发挥重要作用的一个明显例证。我们已经分离出模（mod）的突变体，并表明该基因是一个显性抑制杂色的基因。零突变体是隐性致死性的，具有黑色肿瘤表型。mod蛋白直接结合DNA，这表明它可能锚定多聚体复合物，促进染色质压实和沉默。利用特异性单克隆抗体，免疫细胞化学检测了胚发生过程中mod蛋白的积累模式。该蛋白在所有体细胞核的胚皮细胞化之前被首次检测到，准确地说，是在中心点周围异染色质变得可见的时候。在第一次细胞分裂后，mod蛋白在特定的胚胎原基谱系中表达。基于其产物的显性表型、表达模式和dna结合活性，我们提出mod调节特定细胞系的染色质结构和活性。
Variegation in Drosophila is a manifest illustration of the important role played by chromatin structure in gene expression. We have isolated mutants of modulo (mod) and shown that this gene is a dominant suppressor of variegation. Null mutants are recessive lethal with a melanotic tumour phenotype. The mod protein directly binds DNA, which indicates that it may serve to anchor multimeric complexes promoting chromatin compaction and silencing. Using a specific monoclonal antibody we examined by immunocytochemistry the accumulation pattern of mod protein during embryogenesis. The protein is first detected before the blastoderm cellularization in all somatic nuclei, precisely when pericentromeric heterochromatin becomes visible. After the first cell division, mod protein is expressed in lineages of specific embryonic primordia. Based on its dominant phenotype, expression pattern and DNA-binding activity of its product, we propose that mod regulates chromatin structure and activity in specific cell lineages.