Myelinated mouse nerves studied by X-ray phase contrast zoom tomography
Myelinated mouse nerves studied by X-ray phase contrast zoom tomography
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DOI：
10.1016/j.jsb.2015.11.001
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发表时间：
2015-12-01
影响因子：
3
通讯作者：
Salditt, T.
中科院分区：
文献类型：
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作者：
Bartels, M.;Krenkel, M.;Salditt, T.
我们已经使用X射线相衬断层扫描来解决未切割的，整个有髓视神经，隐神经和坐骨神经的结构。固有电子密度差足以识别轴突结构。通过四氧化锇染色的特异性髓鞘标记能够区分轴突和周围的髓鞘。球面波照明的利用使得能够实现变焦能力，这使得能够对整个坐骨神经间膜进行成像以及识别子结构，例如Ranvier和Schmidt-Lanterman切口的节点。(C)2015 Elsevier Inc. All rights reserved.
We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic intemodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures. (C) 2015 Elsevier Inc. All rights reserved.