Immunolocalization of a 25-kilodalton protein in mouse testis and epididymis.
Immunolocalization of a 25-kilodalton protein in mouse testis and epididymis.
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小鼠睾丸和附睾中 25 千道尔顿蛋白质的免疫定位。
DOI：
10.1095/biolreprod47.5.844
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发表时间：
1992
影响因子：
3.6
通讯作者：
Orgebin-Crist,MC
中科院分区：
文献类型：
--
作者：
Vierula,ME;Araki,Y;Rankin,TL;Tulsiani,DR;Orgebin-Crist,MC
我们最近观察到，针对小鼠附睾腔液蛋白（MEP 9）产生的多克隆抗体识别小鼠睾丸和附睾中的25-kDa抗原[兰金等人，Biol Reprod 1992; 46：747-766]。该抗原通过光镜和电镜免疫组织化学定位。在睾丸中的免疫反应被发现在细长的精子细胞的残留的细胞质中，在残留的身体，并在精子的细胞质滴。在附睾中，上皮主细胞从远端头到远端尾染色。免疫金标记的主细胞呈弥漫性分布，没有优先积累的内吞或分泌器的细胞。在附睾腔，免疫反应仅限于精子胞质液滴。在管腔精子、细胞质液滴或分离的精子质膜中未观察到膜特异性标记。半去势或切断输出管后3周，在附睾中发现免疫反应位点的正常分布。在未成熟小鼠以及附睾中无精子的XXSxr雄性小鼠的附睾上皮中也检测到免疫反应性。这些结果表明，在主细胞中看到的免疫反应性起源于睾丸蛋白质的合成，而不是从破坏的细胞质液滴的内吞作用。此外，这些结果表明，25 kDa的蛋白质是由睾丸和附睾独立合成。
We have recently observed that a polyclonal antibody raised against a mouse epididymal luminal fluid protein (MEP 9) recognizes a 25-kDa antigen in mouse testis and epididymis [Rankin et al., Biol Reprod 1992; 46:747–766]. This antigen was localized by light and electron microscopic immunohistochemistry. The immunoreactivity in the testis was found in the residual cytoplasm of the elongated spermatids, in the residual bodies, and in the cytoplasmic droplets of spermatozoa. In the epididymis, the epithelial principal cells were stained from the distal caput to the distal cauda. Immunogold labeling in the principal cells showed diffuse distribution without preferential accumulation in either the endocytic or the secretory apparatus of the cells. In the epididymal lumen, the immunoreactivity was restricted to the sperm cytoplasmic droplets. No membrane-specific labeling was observed in luminal spermatozoa, cytoplasmic droplets, or isolated sperm plasma membranes. Three weeks after hemicastration or severance of the efferent ducts, a normal distribution of the immunoreactive sites was found in the epididymis. Immunoreactivity, was also detected in the epididymal epithelium of immature mice as well as in that of XXSxrmale mice having no spermatozoa in the epididymis. These results suggest that the immunoreactivity seen in the principal cells originates from synthesis rather than endocytosis of the testicular protein from disrupted cytoplasmic droplets. Furthermore, these results suggest that the 25-kDa protein is synthesized independently by both testis and epididymis.