A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES
A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES
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DOI：
10.1111/j.1432-1033.1969.tb00601.x
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
WESTPHAL, O
中科院分区：
文献类型：
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作者：
GALANOS, C;LUDERITZ, O;WESTPHAL, O
本文介绍了一种从R型细菌中提取脂多糖的方法。萃取混合物为单相，由苯酚、氯仿和石油醚水溶液组成。R形成脂多糖（糖脂）。由于它们的亲脂性，它们完全溶于混合物中。S和T形成脂多糖以及蛋白质、核酸和多糖，是不溶性的，并且它们被排除在提取物之外。该方法是温和的，因为它可以在10°以下进行。产率通常高于通过苯酚-水萃取获得的产率，并且产物通常是水溶性的。迄今为止，已成功地从所有R型细菌中提取脂多糖。
A method is described which is specific for the extraction of lipopolysaccharides from R form bacteria. The extraction mixture which is monophasic, consists of aqueous phenol, chloroform and petroleum ether. R form lipopolysaccharides (glycolipids). due to their lipophilic nature, are completely soluble in the mixture. S and T form lipopolysaccharides as well as proteins, nucleic acids, and polysaccharides, are insoluble, and they are excluded from the extracts. The method is mild, as it can be carried out at below 10°. The yields are generally higher than those obtained by phenol‐water extraction, and the products are usually water‐soluble. Lipopolysaccharides have been successfully extracted from all R form bacteria so far attempted.