Differential sensitivity to pertussis toxin of 3T3 cells transformed with different oncogenes
Differential sensitivity to pertussis toxin of 3T3 cells transformed with different oncogenes
复制标题
不同癌基因转化的3T3细胞对百日咳毒素的敏感性差异
DOI：
10.1016/0014-5793(88)80202-3
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发表时间：
1988
期刊：
影响因子：
3.5
通讯作者：
B. Tocqué
中科院分区：
文献类型：
--
作者：
I. Rey;H. Suarez;F. Lavelle;B. Tocqué
百日咳毒素（PT）可阻断几种G蛋白的活性，已发现对血清或生长因子在3 T3细胞中诱导的DNA合成具有显著的抑制作用。用人类c‐ ras癌基因（Ha‐ras、Ki‐ras、N‐ras）或src（一种编码蛋白激酶的癌基因）转化的3 T3细胞已丧失对PT生长控制的敏感性，尽管PT的底物在体内仍可被ADP-核糖基化。相比之下，用SV 40病毒转化的3 T3细胞在PT降低其生长速率的能力方面表现得像正常的未转化细胞。因此，癌基因可能被分类为PT的“应答者”或“非应答者”。
Pertussis toxin (PT), which blocks the activity of several G‐proteins, has been found to exert a marked inhibitory effect on the DNA synthesis induced in 3T3 cells by serum or growth factors. 3T3 cells transformed with human c‐rasoncogenes (Ha‐ras, Ki‐ras, N‐ras) or withsrc, an oncogene coding for a protein kinase, have lost sensitivity to growth control by PT, even though substrates for PT can still be ADP‐ribosylated in vivo. In contrast, 3T3 cells transformed with the SV40 virus behave like normal untransformed cells with respect to the ability of PT to decrease their growth rate. Oncogenes can thus likely be classified either as ‘responders’ or ‘non‐responders’ to PT.
ras 癌基因对生长因子刺激的磷酸肌醇水解的相反作用：对血小板衍生生长因子的脱敏和对缓激肽的敏感性增强。
DOI：
10.1073/pnas.84.9.2648
发表时间：
1987
影响因子：
11.1
作者：
Parries,G;Hoebel,R;Racker,E
通讯作者：
Racker,E