Age-Related Variation in Cell Density of Human Lumbar Intervertebral Disc
Age-Related Variation in Cell Density of Human Lumbar Intervertebral Disc
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DOI：
10.1097/brs.0b013e3181cd588c
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发表时间：
2011-01-15
期刊：
影响因子：
3
通讯作者：
Boos, Norbert
中科院分区：
文献类型：
--
作者：
Liebscher, Thomas;Haefeli, Mathias;Boos, Norbert
研究设计.在完整的人体运动节段中，系统研究了终板（EP）、髓核（NP）和纤维环（AF）细胞密度在衰老过程中的变化。阐明椎间盘不同解剖区域的细胞密度和总细胞数;测试性别、级别和年龄对细胞密度的影响;并将细胞密度变化与椎间盘退变的组织学体征相关联。关于椎间盘内细胞密度及其年龄相关变化的可用信息很少。然而，这种知识是椎间盘的基于细胞的组织工程方法的关键先决条件。在22例标本的49个完整的腰椎运动节段（新生儿至86岁）的横截面中，通过Abercrombie方法测定EP、NP和AF的细胞密度，并对每个感兴趣区域的细胞总数进行计数。EP、NP和AF的细胞密度从0到16岁显著下降，NP和AF的主要变化发生在0到3岁，此后未观察到显著变化。我们发现细胞密度和组织学变性评分在0和1之间有显著相关性，但评分>1时无相关性。性别和椎间盘水平不影响细胞密度。这项研究提供了关于不同年龄组椎间盘各区域细胞总数的数据。这些知识将有利于基于细胞的治疗方法，这可能会在未来发展。
Study Design. Changes in cell density of endplate (EP), nucleus pulposus (NP), and anulus fibrosus (AF) during ageing were systematically investigated in defined regions of interest in complete human motion segments.Objectives. To elucidate cell density and total cell number in distinct anatomic regions of the intervertebral disc; to test effects of gender, level and age on cell density; and to correlate changes in cell density with histologic signs of disc degeneration.Summary of Background Data. The available information on the cell density within intervertebral discs and its age-related changes is sparse. This knowledge, however, is a crucial prerequisite for cell-based tissue engineering approaches of the intervertebral disc.Methods. In 49 complete cross-sections from lumbar motion segments (newborn to 86 years) from 22 specimens, cell density was determined by the Abercrombie method in EP, NP, and AF, and total cell number was counted per region of interest.Results. Cell density in EP, NP, and AF decreased significantly from 0 to 16 years with the main changes occuring from 0 to 3 years for NP and AF. No significant variations were observed thereafter. We found a significant correlation of cell density and histologic degeneration score between 0 and 1, but not for scores >1. Gender and disc level did not influence cell density.Conclusion. This study provides data concerning the total number of cells in the various regions of the intervertebral disc for different age groups. This knowledge will be beneficial for cell-based treatment approaches, which may evolve in the future.