cDermo-1 misexpression induces dense dermis, feathers, and scales
cDermo-1 misexpression induces dense dermis, feathers, and scales
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DOI：
10.1016/j.ydbio.2004.08.050
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发表时间：
2005-01-01
影响因子：
2.7
通讯作者：
Brand-Saberi, B
中科院分区：
文献类型：
--
作者：
Hornik, C;Krishan, K;Brand-Saberi, B
前表皮和下真皮层间质相互作用是皮肤附属物发育的主要驱动力。羽毛的发育是由羽毛形成皮肤的真皮层发出的一个尚不清楚的信号发起的。外胚层对这一信号的形态学反应是形成一个表皮基板，该基板向间质发出信号，诱导真皮凝聚。表皮和真皮成分共同构成了羽芽的生长。bHLH转录因子cDermo-1在发育中的真皮中表达，是已知最早的未来羽毛束的标记物。为了测试其在羽毛发育过程中的功能，我们使用逆转录病毒表达载体强迫cDermo-1在胚胎鸡真皮中表达。在无羽（翅片）区域，cDermo-1的错误表达导致有羽皮肤（翼片）中常见的致密、增厚的真皮层，并导致间距规则、形状正常的异位羽芽的发育。在翼状动物中，cDermo-1的错误表达促进了羽毛的生长。在后肢皮肤中，根据局部皮肤特征，cDermo-1的错误表达诱导异位鳞片形成。因此，我们发现在发育中的真皮层中强迫cDermo-1表达足以启动导致皮肤附属物形成的发育程序。我们提出cDermo-1在FGF10上游羽毛诱导的初始阶段的作用。(C) 2004爱思唯尔公司版权所有。
Reciprocal epithelio-mesenchymal interactions between the prospective epidermis and the underlying dermis are the major driving forces in the development of skin appendages. Feather development is initiated by a still unknown signal from the dermis in feather-forming skin. The morphological response of the ectoderm to this signal is the formation of an epidermal placode, which signals back to the mesenchyme to induce dermal condensations. Together, epidermal and dermal components constitute the outgrowing feather bud. The bHLH transcription factor cDermo-1 is expressed in developing dermis and is the earliest known marker of prospective feather tracts. To test its function during feather development, we forced cDermo-1 expression in embryonic chicken dermis using a retroviral expression vector. In featherless (apteric) regions, cDermo-1 misexpression induced dense, thickened dermis normally observed in feathered skin (pterylae), and leads to the development of regularly spaced and normally shaped ectopic feather buds. In pterylae, cDermo-1 misexpression enhanced feather growth. In hindlimb skin, according to the local skin identity, misexpression of cDermo-1 induced ectopic scale formation. Thus, we show that forced cDermo-1 expression in developing dermis is sufficient to launch the developmental program leading to skin appendage formation. We propose a role of cDermo-1 at the initial stages of feather induction upstream of FGF10. (C) 2004 Elsevier Inc. All rights reserved.