Isolation and infusion of donor CD34+ bone marrow cells in cadaver kidney transplantation
Isolation and infusion of donor CD34+ bone marrow cells in cadaver kidney transplantation
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DOI：
10.1093/ndt/13.1.34
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发表时间：
1998-01-01
影响因子：
6.1
通讯作者：
Goldman, M
中科院分区：
文献类型：
--
作者：
De Pauw, L;Abramowicz, D;Goldman, M
背景，供体骨髓细胞输注诱导同种异体移植模型耐受。人骨髓中存在的CD 34（+）干细胞具有致耐受性。在手术过程中，将供体CD 34（+）细胞（0.6-3.7 × 10（6）/kg）输注给10名接受OKT 3诱导治疗的肾移植受者。采用巢式PCR检测供者特异性HLA等位基因。输注CD 34（+）干细胞完全耐受。5例患者在术后47-325天随访时仍无急性排斥反应。其他5例患者发生了单次皮质敏感性急性排斥反应。结论：供者CD 34（+）干细胞输注在肾移植中是安全的。该手术的临床实用性仍有待确定。
Background, Infusion of donor bone marrow cells induces tolerance in allograft models. CD34(+) stem cells present in human bone marrow could be endowed with tolerogenic properties.Methods, CD34(+) stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34(+) cells (0.6-3.7 x 10(6)/kg) were infused during surgery in 10 kidney transplant recipients receiving OKT3 as induction therapy. Chimerism was investigated using nested PCR for donor-specific HLA alleles.Results. The infusion of CD34(+) stem cells was perfectly tolerated. Five patients remained free of acute rejection at follow-up, 47-325 days post-operatively. The five other patients underwent a single episode of corticosensitive acute rejection. Long-term chimerism was not induced in the seven patients investigated for the persistence of donor DNA.Conclusions, Infusion of donor CD34(+) stem cells in kidney transplantation is safe. The clinical usefulness of the procedure remains to be established.