High anthocyanin accumulation in an Arabidopsis mutant defective in chloroplast biogenesis
High anthocyanin accumulation in an Arabidopsis mutant defective in chloroplast biogenesis
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叶绿体生物发生缺陷的拟南芥突变体中花青素的高积累
DOI：
10.1007/s10725-019-00481-7
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发表时间：
2019
影响因子：
4.2
通讯作者：
Liu Dong
中科院分区：
文献类型：
--
作者：
Wu Meijia;Lv Xiaolin;Zhou Yingjie;Zeng Yongjun;Liu Dong
花色苷在植物抗氧化胁迫中具有重要的抗氧化保护作用。本研究对具有叶绿体发育缺陷的拟南芥突变株dpg1进行了研究。结果表明，该突变体在苗期花色苷积累增加，花色苷生物合成和调控基因表达上调。进一步研究表明，外源ABA(脱落酸)处理显著促进了dpg1突变体的叶绿体发育，花青素含量显著下降至野生型水平。用去甲氟拉松模拟野生型拟南芥的氧化胁迫处理时，花色苷含量较对照显著增加。而外源ABA处理能显著降低氧化胁迫诱导的花色苷积累水平。此外，ABA信号通路中的ABI1(脱落酸不敏感1)和ABI3(脱落酸不敏感3)被发现在这一过程中起着重要作用。这些结果表明，dpg1突变体幼苗花青素积累的增加可能是由氧化胁迫介导的。同时，ABI1和ABI3参与了ABA抑制氧化胁迫诱导的花青素积累的过程。
Anthocyanin has an important antioxidant protective effect on plant resistance to oxidative stress. In this study, an Arabidopsis mutantdpg1(delayed pale-greening) with a chloroplast development defect was studied. It was found that the anthocyanin accumulation of this mutant had increased during the seedling stage, and the expressions of the anthocyanin biosynthetic and regulatory genes were up-regulated. Further studies showed that exogenous ABA (abscisic acid) treatments significantly promoted the chloroplast development of thedpg1mutant, and the anthocyanin content was significantly decreased to the level of the wild-type. When using NF (norflurazon) to simulate the oxidative stress treatments of wild-type Arabidopsis, the anthocyanin content had significantly increased compared with the control. However, the exogenous ABA treatments could significantly reduce the anthocyanin accumulation level induced by the oxidative stress. Furthermore, the components ABI1 (abscisic acid insensitive 1) and ABI3 (abscisic acid insensitive 3) of the ABA signaling pathway were found to play important roles during this process. These results indicate that the increases in the anthocyanin accumulation in thedpg1mutant seedlings could be mediated by oxidative stress. Meanwhile, the ABI1 and ABI3 were involved in the process of the ABA inhibiting anthocyanin accumulation which had been induced by the oxidative stress.