Mycelium-bound lipase production from Aspergillus niger MYA 135, and its potential applications for the transesterification of ethanol
Mycelium-bound lipase production from Aspergillus niger MYA 135, and its potential applications for the transesterification of ethanol
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DOI：
10.1002/jobm.201000232
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发表时间：
2011-06-01
影响因子：
3.1
通讯作者：
Maria Pera, Licia
中科院分区：
文献类型：
--
作者：
Leticia Colin, Veronica;Domingo Baigori, Mario;Maria Pera, Licia
对尼日尔MYA 135组成型和诱导型脂肪酶的生物技术应用潜力进行了评价。为此，研究了环境条件对该菌株菌丝体结合脂肪酶生产的影响，无论是在不存在还是存在2%橄榄油的情况下培养。据报道，尼日尔MYA 135的菌丝体结合脂肪酶在含有乙醇的反应混合物中具有高稳定性;这对于它们在生物柴油合成中的应用可能是特别重要的。在这方面，脂肪酶来源的乙醇酯交换反应中产生的对硝基苯基棕榈酸酯作为酰基donors.Under我们的测定条件下，在没有或存在的橄榄油的情况下产生的菌丝体的水解和合成活性的性能也explored. Under我们的测定条件下，没有高度相关性。虽然通过向培养基中添加脂质强烈增加了水解活性，但在乙醇的酯交换反应中的最佳性能与在不存在橄榄油的情况下产生的菌丝体相关。有趣的是，与先前报道的该菌株的活性相比，用Fe+3补充培养基使酯交换活性增加了71%。因此，尼日尔MYA 135的组成型脂肪酶来源被认为是有前途的工业生物柴油燃料生产。
The potential biotecnological applications of both constitutive and inducible lipase sources from Aspergillus niger MYA 135 were evaluated. To this end, the effect of environmental conditions on mycelium-bound lipase production from this strain was studied, when cultured either in the absence or presence of 2% olive oil. It was previously reported that mycelium-bound lipase from Aspergillus niger MYA 135 possess high stability in reaction mixtures containing ethanol; which could be especially important for their use in biodiesel synthesis. In this connection, the performance of the lipase sources produced in the transesterification of ethanol using p-nitrophenyl palmitate as acyl donor was also explored.Under our assay conditions, hydrolytic and synthetic activity of the mycelia produced in the absence or presence of olive oil were not highly correlated. While the hydrolytic activity was strongly increased by the addition of lipid to the culture medium, the best performance in the transesterification reactions of ethanol were associated with mycelia produced in absence of olive oil. Interestingly, the supplementation of the culture medium with Fe+3 increased the transesterification activity by 71%, as compared to the activity previously reported for this strain. Therefore, the constitutive lipase sources from Aspergillus niger MYA 135 are considered to be promising for industrial biodiesel-fuel production.