Preparation, Optimization and Characterization of Bovine Lactoferrin‐loaded Liposomes and Solid Lipid Particles Modified by Hydrophilic Polymers Using Factorial Design
Preparation, Optimization and Characterization of Bovine Lactoferrin‐loaded Liposomes and Solid Lipid Particles Modified by Hydrophilic Polymers Using Factorial Design
复制标题
采用析因设计制备牛乳铁蛋白脂质体和亲水聚合物修饰的固体脂质颗粒，优化和表征
DOI：
10.1111/cbdd.12269
复制
发表时间：
2014
影响因子：
3
通讯作者：
Jingyuan Wen
中科院分区：
文献类型：
--
作者：
Xudong Yao;C. Bunt;J. Cornish;S. Quek;Jingyuan Wen
采用24全因子设计，制备了用于口服乳铁蛋白（bLf）的生物粘附脂质体和固体脂质颗粒（SLPs），以确定影响颗粒大小和药物包封效率（EE）的关键配方变量。用扫描电子显微镜观察了由嵌入多个粒子的聚合物网络组成的聚合物-颗粒混合物的网状结构。傅里叶红外光谱（FTIR）证实了bLf包封在果胶和壳聚糖修饰脂质体和slp后的化学稳定性。牛乳铁蛋白位于磷脂双分子层内，而SLPs中bLf位于基质内。通过载药颗粒的差示扫描量热法（DSC）研究了包封后bLf的结晶性质，表明bLf在果胶和壳聚糖修饰的脂质体和slp的聚合物脂质基质中呈无定形分散。果胶修饰脂质体和壳聚糖修饰脂质体和slp中bLf的体内药代动力学研究表明，与游离bLf相比，bLf在大鼠血液中的平均停留时间（MRT）延长，相对生物利用度（Fbio%）提高1.95 ~ 2.69倍。所开发的载体系统被认为是有前途的口服给药载体。
Bioadhesive liposomes and solid lipid particles (SLPs) modified by pectin and chitosan for oral administration of bovine lactoferrin (bLf) were prepared using a 24 full‐factorial design to identify the key formulation variables influencing particle size and drug entrapment efficiency (EE). Netlike structures of the polymer–particle mixture consisting of a polymeric network in which multiple particles were imbedded were observed by scanning electron microscopy (SEM). Chemical stability of bLf after encapsulation into pectin‐ and chitosan‐modified liposomes and SLPs was confirmed by Fourier transform infrared spectra (FTIR). Bovine lactoferrin was located within phospholipid bilayer, whereas in SLPs bLf was within the matrix. The crystalline nature of bLf after encapsulation was investigated by differential scanning calorimetry (DSC) of drug‐loaded particles, indicating amorphous dispersion of bLf in the polymer–lipid matrix of pectin‐ and chitosan‐modified liposomes and SLPs. In vivo pharmacokinetic investigation of bLf in pectin‐ and chitosan‐modified liposomes and SLPs showed prolonged mean residence time (MRT) of bLf in rat blood and increased the relative bioavailability (Fbio%) by 1.95‐ to 2.69‐fold compared with free bLf. The developed carrier systems are considered to be promising vehicles for oral delivery.
影响因子：
5.6
作者：
Moore, SA;Anderson, BF;Baker, EN
通讯作者：
Baker, EN
影响因子：
5.6
作者：
ANDERSON, BF;BAKER, HM;BAKER, EN
通讯作者：
BAKER, EN