A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp durum)
A multiparental cross population for mapping QTL for agronomic traits in durum wheat (Triticum turgidum ssp durum)
复制标题
DOI：
10.1111/pbi.12424
复制
发表时间：
2016-02-01
影响因子：
13.8
通讯作者：
Salvi, Silvio
中科院分区：
文献类型：
--
作者：
Milner, Sara Giulia;Maccaferri, Marco;Salvi, Silvio
多亲本杂交设计为数量性状位点定位提供了一种有效的替代方法，因为它们具有更广泛的遗传基础和更高的定位分辨率。我们描述了硬粒小麦（Triticum turgidum ssp）重组自交系（RIL）群体的发展和部署。由四个优良品种杂交而得。通过对338个ril进行基因分型，得到了全长2664cM、包含7594个单核苷酸多态性（snp）的连锁图谱。采用方正单倍型概率区间作图和抽穗期、成熟期、株高、产量的SNP双等位基因检测进行QTL分析。在不同的环境和检测方法中鉴定出16个QTL，包括2BL和7AS染色体上的两个产量QTL，前者与光周期响应基因Ppd-B1独立定位，后者与春化位点VRN-A3重叠。此外，还发现了21个具有环境特异性效应的QTL。结果表明，环境特异性QTL普遍存在，但对粮食产量的控制作用相对较小。所有性状的QTL等位基因在亲本间的遗传效应方向和大小均存在功能差异。结果表明，在已知抽穗日期位点上，基于创始人单倍型的QTL结果与功能性等位基因高度匹配。尽管有四位创始人，每个QTL平均只估计到2.1种不同的功能单倍型。该硬粒小麦群体为育种计划中典型精英背景的农艺性状的详细遗传解剖提供了一个图谱资源。
Multiparental cross designs for mapping quantitative trait loci (QTL) provide an efficient alternative to biparental populations because of their broader genetic basis and potentially higher mapping resolution. We describe the development and deployment of a recombinant inbred line (RIL) population in durum wheat (Triticum turgidum ssp. durum) obtained by crossing four elite cultivars. A linkage map spanning 2664cM and including 7594 single nucleotide polymorphisms (SNPs) was produced by genotyping 338 RILs. QTL analysis was carried out by both interval mapping on founder haplotype probabilities and SNP bi-allelic tests for heading date and maturity date, plant height and grain yield from four field experiments. Sixteen QTL were identified across environments and detection methods, including two yield QTL on chromosomes 2BL and 7AS, with the former mapped independently from the photoperiod response gene Ppd-B1, while the latter overlapped with the vernalization locus VRN-A3. Additionally, 21 QTL with environment-specific effects were found. Our results indicated a prevalence of environment-specific QTL with relatively small effect on the control of grain yield. For all traits, functionally different QTL alleles in terms of direction and size of genetic effect were distributed among parents. We showed that QTL results based on founder haplotypes closely matched functional alleles at known heading date loci. Despite the four founders, only 2.1 different functional haplotypes were estimated per QTL, on average. This durum wheat population provides a mapping resource for detailed genetic dissection of agronomic traits in an elite background typical of breeding programmes.