EFFECTS OF OXYGEN STRESS ON MEMBRANE FUNCTIONS IN ESCHERICHIA-COLI - ROLE OF HPI CATALASE
EFFECTS OF OXYGEN STRESS ON MEMBRANE FUNCTIONS IN ESCHERICHIA-COLI - ROLE OF HPI CATALASE
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DOI：
10.1128/jb.170.4.1837-1842.1988
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发表时间：
1988-04-01
影响因子：
3.2
通讯作者：
KOGOMA, T
中科院分区：
文献类型：
--
作者：
FARR, SB;TOUATI, D;KOGOMA, T
采用不同的氧化胁迫条件，研究了它们对大肠杆菌K-12细胞膜转运的影响。氧化条件包括H2 O2、白花丹素（一种产生超氧自由基[O2-]的氧化还原循环化合物）和增加的氧分压。超氧自由基产生条件和H2 O2处理被发现导致质子动力依赖和非依赖运输的快速下降。H_2O_2预处理的细胞有能力迅速恢复质子动力依赖和非依赖运输。诱导需要转录和翻译，并依赖于oxyR+和katG+，提供证据表明，这些基因在运输的快速恢复中起着至关重要的作用。研究了氧化引起的质子动力损失对细胞生长和大分子合成的影响。
Different conditions of oxidative stress were used to study their effects on membrane transport in Escherichia coli K-12. The oxidizing conditions included H2O2, plumbagin (a redox cycling compound that generates superoxide radicals [O2-]), and increased partial pressure of oxygen. Both superoxide radical-generating conditions and H2O2 treatments were found to cause a rapid decrease in proton motive force-dependent and -independent transport. H2O2-pretreated cells had the ability to rapidly recover proton motive force-dependent and -independent transport. The induction required transcription and translation and was dependent on oxyR+ and katG+, providing evidence that these genes play crucial roles in the rapid recovery of transport. The effects of oxidatively induced loss of proton motive force on cell growth and macromolecular synthesis were also investigated.