GROWTH FAILURE AND DECREASED BONE-MINERAL OF NEWBORN RATS WITH CHRONIC FUROSEMIDE THERAPY
GROWTH FAILURE AND DECREASED BONE-MINERAL OF NEWBORN RATS WITH CHRONIC FUROSEMIDE THERAPY
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DOI：
10.1203/00006450-198601000-00021
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发表时间：
1986-01-01
影响因子：
3.6
通讯作者：
NEUMANN, V
中科院分区：
文献类型：
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作者：
KOO, WWK;GUAN, ZP;NEUMANN, V
为了检验在其他健康的新生动物中慢性呋塞米治疗可能导致骨矿物质[钙（CA）和镁（Mg）]含量降低的假设，将每窝Sprague-Dawley大鼠幼仔中的健康同窝仔随机分配至3组：对照组、低剂量呋塞米（5 mg/kg/天）和高剂量呋塞米（15 mg/kg/天）。幼仔在出生后第4 - 28天接受给药。肾脏和胫骨的湿重和干重与处死时的体重显著相关。呋塞米处理的幼仔表现出剂量依赖性生长延迟，总骨（骨）钙和镁降低，尿钙和镁浓度增加，骨钙和尿钙浓度之间呈显著负相关。当骨钙和镁标准化为每克骨干重时，各组间无显著差异。各组之间在骨磷或尿磷浓度;肾脏和血清钙和镁;或血清钠、钾、碱性磷酸酶和免疫反应性甲状旁腺激素浓度方面无显著差异。我们的结论是，慢性呋塞米治疗导致生长障碍和增加尿钙和镁的损失。总骨钙和镁在呋塞米治疗的幼崽减少成比例的生长迟缓，但每单位干重的骨矿物质含量保持类似于对照组幼崽。
To test the hypothesis that chronic furosemide treatment in otherwise healthy newborn animals may lead to lowered bone mineral [calcium (CA) and magnesium (Mg)] content, healthy littermates within each litter of Sprague-Dawley rat pups were randomly assigned to three groups: control, low dose furosemide (5 mg/kg/day), and high dose furosemide (15 mg/kg/day). The pups were treated between days 4 and 28 postnatally. The wet and dry weights of kidneys and tibiae significantly correlated with body weights at sacrifice. Furosemide-treated pups demonstrated a dose-dependent growth delay, decreased total bone (tibiae) Ca and Mg, increased urine Ca and Mg concentration, and a significant inverse correlation between bone Ca and urine Ca concentration. There was no significant difference among the groups when bone Ca and Mg were normalized to per gram of bone dry weight. There were no significant differences among the groups with respect to bone phosphorus or urinary phosphorus concentration; kidney and serum Ca and Mg; or serum sodium, potassium, alkaline phosphatase and immunoreactive parathyroid hormone concentration. We conclude that chronic furosemide therapy leads to growth failure and to increased urinary losses of Ca and Mg. Total bone Ca and Mg in the furosemide-treated pups were diminished in proportion to growth retardation but the bone mineral content per unit of dry weight remained similar to control pups.