Isolation and identification of age-related DNA methylation markers for forensic age-prediction
Isolation and identification of age-related DNA methylation markers for forensic age-prediction
复制标题
用于法医年龄预测的年龄相关 DNA 甲基化标记的分离和鉴定
DOI：
10.1016/j.fsigen.2014.03.006
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发表时间：
2014-07-01
影响因子：
3.1
通讯作者：
Huang, Dai Xin
中科院分区：
文献类型：
--
作者：
Yi, Shao Hua;Xu, Long Chang;Huang, Dai Xin
犯罪预测是法庭科学的重要组成部分。对于犯罪现场的一般法医生物样本，目前还没有一种有效的个体年龄预测方法。越来越多的证据表明，衰老类似于一个发育调控的过程，受到人类基因组中存在的特定年龄相关甲基化的严格控制。本研究分离并鉴定了40名献血者血液中胞嘧啶甲基化程度与年龄显著相关的8个基因片段。此外，我们验证了每个基因片段的两个CpG位点，并在40名男性和25名女性的广泛年龄范围（11-72岁）的一般人群样本中复制了我们的结果。这些片段的甲基化在六十年的范围内与年龄呈线性关系（片段P1（r = -0.64）、P2（r = -0.58）、P3（r = -0.79）、R1（r = 0.82）、R2（r = 0.63）、R3（r = 0.59）、R4（r = 0.63）和R5（r = 0.62））。使用每个片段的两个CpG位点的平均甲基化，我们建立了一个回归模型，解释了95%的年龄方差，并且能够非常准确地预测个体的年龄（R2 = 0.918）。预测值与样本中观察到的年龄高度相关（r = 0.91）。本研究提示DNA甲基化可能成为预测年龄的一个有效的生物学指标。此外，基因组中相关位点的测量可以作为常规法医筛查的工具，以预测生物样本的年龄。(c)2014爱思唯尔爱尔兰有限公司版权所有。
Age-prediction is an important part of forensic science. There is no available method of individual age-prediction for general forensic biological samples at crime scenes. Accumulating evidence indicates that aging resembles a developmentally regulated process tightly controlled by specific age-associated methylation exists in human genome. This study isolated and identified eight gene fragments in which the degree of cytosine methylation is significantly correlated with age in blood of 40 donors. Furthermore, we validated two CpG sites of each gene fragment and replicated our results in a general population sample of 40 males and 25 females with a wide age-range (11-72 years). The methylation of these fragments is linear with age over a range of six decades (Fragment P1 (r = -0.64), P2 (r = -0.58), P3 (r = -0.79), R1 (r = 0.82), R2 (r = 0.63), R3 (r = 0.59), R4 (r = 0.63) and R5 (r = 0.62)). Using average methylation of two CpG sites from each fragment, we built a regression model that explained 95% of the variance in age and is able to predict the age of an individual with great accuracy (R2 = 0.918). The predicted values are highly correlated with the observed age in the sample (r = 0.91). This study implicates that DNA methylation will be an available biological marker of age-prediction. Furthermore, measurement of relevant sites in the genome could be a tool in routine forensic screening to predict age of biological samples. (c) 2014 Elsevier Ireland Ltd. All rights reserved.