RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice
RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice
复制标题
RNA干扰敲低氨肽酶N基因降低二化螟幼虫对表达Cry1Ab/Cry1Ac和Cry1Ca的转基因水稻的敏感性
DOI：
10.1016/j.jip.2017.03.001
复制
发表时间：
2017
影响因子：
3.4
通讯作者：
Ma Weihua
中科院分区：
文献类型：
--
作者：
Qiu Lin;Fan Jinxing;Zhang Boyao;Liu Lang;Wang Xiaoping;Lei Chaoliang;Lin Yongjun;Ma Weihua
表达苏云金芽孢杆菌（Bt）Cry毒素的转基因水稻对鳞翅目害虫具有抗性，例如亚洲水稻的主要害虫二化螟。了解这些毒素如何与其宿主相互作用对于了解其杀虫作用至关重要。在本研究中，通过RNA干扰技术敲低两个氨肽酶N基因（APN 1和APN 2）导致了C.二化螟幼虫对Bt水稻品种TT 51（Cry 1AbandCry 1Acfusion基因）和T1 C-19（Cry 1Ca基因）的抗性显著，而对T2 A-1（Cry 2Aa基因）的抗性不显著。这表明APN 1和APN 2是Cry 1A和Cry 1C毒素在C中的受体。suppressalis.
Transgenic rice expressingBacillus thuringiensis(Bt) Cry toxins are resistant to lepidopteran pests, such asChilo suppressalis, a major insect pest of rice in Asia. Understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. In this study, knockdown of two aminopeptidase N genes (APN1 and APN2) by RNA interference resulted in decreased susceptibility ofC. suppressalislarvae to the Bt rice varieties TT51 (Cry1AbandCry1Acfusion genes) and T1C-19 (Cry1Ca), but not T2A-1 (Cry2Aa). This suggests that APN1 and APN2 are receptors for Cry1A and Cry1C toxins inC. suppressalis.