Elevating the vitamin E content of plants through metabolic engineering
Elevating the vitamin E content of plants through metabolic engineering
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DOI：
10.1126/science.282.5396.2098
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发表时间：
1998-12-11
期刊：
影响因子：
56.9
通讯作者：
DellaPenna, D
中科院分区：
文献类型：
--
作者：
Shintani, D;DellaPenna, D
α-生育酚（维生素E）是一种脂溶性抗氧化剂，仅由光合生物合成。α-生育酚是哺乳动物饮食的基本成分，并且超过美国推荐的每日允许量的摄入量与许多退化性人类疾病的发病率降低相关。植物油是生育酚的主要膳食来源，通常含有α-生育酚作为次要组分和高水平的其生物合成前体γ-生育酚。使用基于基因组学的方法克隆α-生育酚合成中的最终酶γ-生育酚甲基转移酶。γ-生育酚甲基转移酶在拟南芥种子中的过表达有利于α-生育酚的油成分。农业油料作物的类似增加将增加美国平均饮食中的维生素E水平。
alpha-Tocopherol (vitamin E) is a Lipid-soluble antioxidant synthesized only by photosynthetic organisms. alpha-Tocopherol is an essential component of mammalian diets, and intakes in excess of the U.S. recommended daily allowance are correlated with decreased incidence of a number of degenerative human diseases. Plant oils, the main dietary source of tocopherols, typically contain alpha-tocopherol as a minor component and high Levels of its biosynthetic precursor, gamma-tocopherol, A genomics-based approach was used to clone the final enzyme in alpha-tocopherol synthesis, gamma-tocopherol methyltransferase. Overexpression of gamma-tocopherol methyltransferase in Arabidopsis seeds shifted oil compositions in favor of alpha-tocopherol. Similar increases in agricultural oil crops would increase vitamin E Levels in the average U.S. diet.