Effective Antimicrobial Activity of Plectasin-Derived Antimicrobial Peptides against Staphylococcus aureus Infection in Mammary Glands.
Effective Antimicrobial Activity of Plectasin-Derived Antimicrobial Peptides against Staphylococcus aureus Infection in Mammary Glands.
复制标题
菌丝菌素衍生抗菌肽对乳腺金黄色葡萄球菌感染的有效抗菌活性
DOI：
10.3389/fmicb.2017.02386
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Li L;Wang L;Gao Y;Wang J;Zhao X
金黄色葡萄球菌（S. aureus）是多种疾病的病原体，从轻微的皮肤感染到危及生命的疾病。细菌对抗生素产生耐药性，导致许多抗生素失效。众所周知，菌丝菌素衍生的抗菌肽（AMP；NZ2114 和 MP1102）是有前景的抗生素替代品。然而，它们对乳腺中金黄色葡萄球菌的活性尚不清楚。我们的目的是评估 NZ2114 和 MP1102 对牛奶、培养乳腺上皮细胞和小鼠模型中金黄色葡萄球菌的抗菌活性，以评估它们作为抗乳腺炎药物的潜力。 NZ2114和MP1102在体外对培养基和牛奶中的金黄色葡萄球菌具有杀菌作用。浓度为 100 μg/mL 的 NZ2114 和 MP1102 在 4 小时内将加工牛乳中的金黄色葡萄球菌数量减少了几乎 100%。同样，NZ2114 和 MP1102 均能有效减少培养的乳腺上皮细胞中内化的金黄色葡萄球菌的数量。最后，与小鼠模型中的缓冲液对照相比，两种 AMP 均显着降低了乳腺中金黄色葡萄球菌的负荷以及 TNF-α 和 IL-6 的浓度。我们的结果表明 NZ2114 和 MP1102 可用于治疗金黄色葡萄球菌引起的乳腺炎。
Staphylococcus aureus (S. aureus) is the causative agent for a wide variety of illnesses ranging from minor skin infections to life-threatening diseases. Development of antibiotic resistance by the bacteria has rendered many antibiotics ineffective. It has been known that plectasin-derived antimicrobial peptides (AMPs; NZ2114 and MP1102) are promising alternatives to antibiotics. However, their activities against S. aureus in mammary glands were unknown. Our objective was to assess the antimicrobial activities of NZ2114 and MP1102 against S. aureus in milk, in cultured mammary epithelial cells, and in a mouse model in order to evaluate their potentials as anti-mastitis agents. NZ2114 and MP1102 showed in vitro bactericidal effects against S. aureus in both the culture medium and the milk. NZ2114 and MP1102 at the concentration of 100 μg/mL reduced the number of S. aureus by almost 100% within 4 h in processed bovine milk. Similarly, both NZ2114 and MP1102 were efficient to reduce the number of internalized S. aureus in cultured mammary epithelial cells. Finally, both AMPs significantly reduced the S. aureus load and concentrations of TNF-α and IL-6 in mammary glands, compared to a buffer control in the mouse model. Our results suggest that NZ2114 and MP1102 may be used to treat S. aureus-induced mastitis.