Serum microRNA‐124 is a novel biomarker for liver necroinflammation in patients with chronic hepatitis B virus infection
Serum microRNA‐124 is a novel biomarker for liver necroinflammation in patients with chronic hepatitis B virus infection
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DOI：
10.1111/jvh.12284
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发表时间：
2015-02
影响因子：
2.5
通讯作者：
J.-Y. Wang;R. Mao;Y.‐M. Zhang;Y.‐J. Zhang;H-Y Liu;Y.-L Qin;M.-J Lu;J-M Zhang
中科院分区：
文献类型：
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作者：
J.-Y. Wang;R. Mao;Y.‐M. Zhang;Y.‐J. Zhang;H-Y Liu;Y.-L Qin;M.-J Lu;J-M Zhang
慢性B型肝炎病毒（HBV）感染且转氨酶正常或轻度升高的患者可能存在持续的显著肝损伤，如肝活检所证实。然而，没有合适的非侵入性的方法来识别这些患者的肝脏坏死性炎症。我们的目的是研究microRNA-124作为肝脏坏死性炎症的新生物标志物的能力。共招募了131例慢性HBV感染患者进行肝活检，以进行坏死性炎症分级（G）和纤维化分期（S）。30名健康个体被包括作为对照（HC）。使用qRT-PCR测量血清microRNA-124和microRNA-122水平。来自研究人群的45名接受恩替卡韦治疗的患者监测了与肝脏组织学改善相关的血清microRNA-124水平的变化。血清microRNA-124水平在区分肝脏坏死性炎症等级方面的能力与丙氨酸氨基转移酶（ALT）进行了比较，并进行了肝活检验证。慢性HBV感染患者的血清microRNA-124水平显著高于HC（P 104拷贝/mL，血清microRNA-124水平的受试者工作特征（ROC）曲线产生的ROC曲线下面积（AUC）为0.840，区分中度至重度肝脏坏死性炎症（G ≥ 2）的灵敏度为58.3%，特异性为91.7%）。
Patients with chronic hepatitis B virus (HBV) infection and normal or mildly increased transaminases may have sustained significant liver damage, as verified by liver biopsy. However, no suitable noninvasive method exists for identifying liver necroinflammation in such patients. We aimed to investigate the power of microRNA‐124 as a novel biomarker for liver necroinflammation. A total of 131 recruited patients with chronic HBV infection underwent liver biopsy for grading of necroinflammation (G) and staging of fibrosis (S). Thirty healthy individuals were included as controls (HCs). Serum microRNA‐124 and microRNA‐122 levels were measured using qRT‐PCR. Forty‐five patients from the study population receiving entecavir therapy were monitored for changes in serum microRNA‐124 levels in association with improved liver histology. The capacity of serum microRNA‐124 levels in discriminating the grade of liver necroinflammation was compared with alanine aminotransferase (ALT) with liver biopsy validation. Serum microRNA‐124 levels were significantly higher in patients with chronic HBV infection than in HCs (P 104 copies/mL, receiver operating characteristic (ROC) curve of serum microRNA‐124 levels yielded an area under ROC curve (AUC) of 0.840, with 58.3% sensitivity and 91.7% specificity in discriminating between moderate‐to‐severe liver necroinflammation (G ≥ 2).