AUTORADIOGRAPHIC ANALYSIS OF AGE-DEPENDENT CHANGES IN SEROTONIN 5-HT2 RECEPTORS OF THE HUMAN BRAIN POSTMORTEM
AUTORADIOGRAPHIC ANALYSIS OF AGE-DEPENDENT CHANGES IN SEROTONIN 5-HT2 RECEPTORS OF THE HUMAN BRAIN POSTMORTEM
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DOI：
10.1016/0006-8993(90)90081-l
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发表时间：
1990-06-11
期刊：
影响因子：
2.9
通讯作者：
BIEGON, A
中科院分区：
文献类型：
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作者：
GROSSISSEROFF, R;SALAMA, D;BIEGON, A
对人脑中5-HT2受体的放射自显影分析，以[~3H]酮丝氨酸为配基，揭示了受体标记随年龄变化的区域特异性变化。在12名正常受试者的前额叶皮质和海马齿状回，标记密度在第2和第3年随年龄急剧下降，在50岁左右达到最低，然后在第6和第7年又开始增加。其他被研究的大脑区域，包括额顶和颞叶皮质、基底节和丘脑，没有显示出随年龄增长的显著变化。23例正常人前牙周皮质标本的饱和结合实验表明，标记密度的降低是由于受体密度(Bmax)的变化，而亲和力(Kd)没有明显变化。性别、酒精存在和死后延迟对酮丝氨酸的结合没有影响。
Autoradiographic analysis of 5-HT2 receptors in the human brain, using [3H]ketanserin as a ligand, reveals region-specific changes in receptor labeling as a function of age. In the prefrontal cortex and hippocampal dentate gyrus of 12 normal subjects, label density decreases sharply with age over the 2nd and 3rd decades, reaches a minimum around age 50 and then starts to increase again in the 6th and 7th decades. Other brain regions studied, including frontoparietal and temporal cortex, basal ganglia and thalamus, did not show significant changes with age. Saturation binding experiments on prefontal cortical samples from 23 normal subjects reveal that the decrease in label density is due to changes in receptor density (Bmax) with no apparent change in affinity (Kd). Sex, presence of alcohol and postmortem delay had no effect on ketanserin binding.