Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens
Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens
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DOI：
10.1186/s13568-019-0914-2
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发表时间：
2019-11-21
期刊：
影响因子：
3.7
通讯作者：
Cheng, Yeong-Hsiang
中科院分区：
文献类型：
--
作者：
Horng, Yi-Bing;Yu, Yu-Hsiang;Cheng, Yeong-Hsiang
猪痢疾和坏死性肠炎是全世界畜牧业的一大祸害。一些国家已经禁止在动物生产中使用抗生素作为生长促进剂。表面素是抗生素和抗菌剂的潜在替代品。然而，芽孢杆菌衍生的表面素对水痢短螺旋体和产气荚膜梭菌的抑菌活性尚不清楚。本研究研究了枯草芽孢杆菌和地衣芽孢杆菌生产的表面素对水痢杆菌和产气荚膜芽孢杆菌的抑菌作用。结果表明，枯草芽孢杆菌中检测到多种表面素异构体，而地衣芽孢杆菌发酵产物中仅检测到一种表面素异构体。与地衣芽孢杆菌相比，枯草芽孢杆菌生产的表面素对水痢疾杆菌具有显著的抗菌活性。枯草芽孢杆菌衍生的表面素能抑制细菌生长并破坏水痢芽孢杆菌的形态。此外，枯草芽孢杆菌产生的表面素对产气荚膜芽孢杆菌的生长具有最高的抑制活性。地衣芽孢杆菌发酵产物中提取的表面素对产气荚膜芽孢杆菌具有较强的抑菌活性。这些结果共同表明，芽孢杆菌衍生的表面素具有开发作为饲料添加剂的潜力，并可作为抗生素的替代品，用于预防动物工业中的水痢杆菌和产气荚膜芽孢杆菌相关疾病。
Swine dysentery and necrotic enteritis are a bane to animal husbandry worldwide. Some countries have already banned the use of antibiotics as growth promoters in animal production. Surfactin is a potential alternative to antibiotics and antibacterial agents. However, the antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens are still poorly understood. In the current study, the antibacterial effects of surfactin produced from Bacillus subtilis and Bacillus licheniformis on B. hyodysenteriae and C. perfringens were evaluated. Results showed that multiple surfactin isoforms were detected in B. subtilis, while only one surfactin isoform was detected in B. licheniformis fermented products. The surfactin produced from B. subtilis exhibited significant antibacterial activity against B. hyodysenteriae compared with surfactin produced from B. licheniformis. B. subtilis-derived surfactin could inhibit bacterial growth and disrupt the morphology of B. hyodysenteriae. Furthermore, the surfactin produced from B. subtilis have the highest activity against C. perfringens growth. In contrast, B. licheniformis fermented product-derived surfactin had a strong bacterial killing activity against C. perfringens compared with surfactin produced from B. subtilis. These results together suggest that Bacillus species-derived surfactin have potential for development as feed additives and use as a possible substitute for antibiotics to prevent B. hyodysenteriae and C. perfringens-associated disease in the animal industry.