Zebrafish facial lymphatics develop through sequential addition of venous and non-venous progenitors
Zebrafish facial lymphatics develop through sequential addition of venous and non-venous progenitors
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DOI：
10.15252/embr.201847079
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发表时间：
2019-05-01
期刊：
影响因子：
7.7
通讯作者：
Astin, Jonathan W.
中科院分区：
文献类型：
--
作者：
Eng, Tiffany C. Y.;Chen, Wenxuan;Astin, Jonathan W.
已知淋巴管来源于静脉;然而，最近的谱系追踪实验提出，特定的淋巴网络可能起源于静脉和非静脉来源。尽管如此，非静脉淋巴祖细胞的直接证据仍然缺失。在这里，我们表明，斑马鱼面部淋巴网络是来自三个不同的祖细胞群体，依次添加到发展中的面部淋巴通过中继样机制。我们发现，虽然两个面部淋巴祖细胞的人口是静脉的起源，第三个人口，称为腹主动脉淋巴管母细胞（VA-L），不发芽从血管，相反，它产生于一个迁移成血管细胞腹主动脉附近，最初缺乏静脉和淋巴标志物，并有助于面部血管和下鳃动脉。我们建议，静脉和非静脉祖细胞的顺序添加允许面部神经元形成在一个区域，是相对缺乏静脉。总的来说，这项研究提供了确凿的，基于实时成像的非静脉淋巴祖细胞的证据，并表明淋巴管的起源和发育是依赖于环境的。
Lymphatic vessels are known to be derived from veins; however, recent lineage-tracing experiments propose that specific lymphatic networks may originate from both venous and non-venous sources. Despite this, direct evidence of a non-venous lymphatic progenitor is missing. Here, we show that the zebrafish facial lymphatic network is derived from three distinct progenitor populations that add sequentially to the developing facial lymphatic through a relay-like mechanism. We show that while two facial lymphatic progenitor populations are venous in origin, the third population, termed the ventral aorta lymphangioblast (VA-L), does not sprout from a vessel; instead, it arises from a migratory angioblast cell near the ventral aorta that initially lacks both venous and lymphatic markers, and contributes to the facial lymphatics and the hypobranchial artery. We propose that sequential addition of venous and non-venous progenitors allows the facial lymphatics to form in an area that is relatively devoid of veins. Overall, this study provides conclusive, live imaging-based evidence of a non-venous lymphatic progenitor and demonstrates that the origin and development of lymphatic vessels is context-dependent.