Inheritance of the resistance to Macrophomina phaseolina and identification of RAPD markers linked to the resistance genes in beans
Inheritance of the resistance to Macrophomina phaseolina and identification of RAPD markers linked to the resistance genes in beans
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DOI：
10.1094/phyto-86-674
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发表时间：
1996-06-01
期刊：
影响因子：
3.2
通讯作者：
Weeden, NF
中科院分区：
文献类型：
--
作者：
Olaya, G;Abawi, GS;Weeden, NF
采用传统方法和分子标记法对菜绿巨褐虫的抗性遗传进行了研究。遗传研究是基于抗性菌株BAT-477和易感菌株a -70之间的杂交。用菜籽病菌核侵染土壤接种大豆种子，研究了其对菜籽病的抗性。在F-2群体中，抗性植株与感感植株的比例接近9:7。散装分离分析鉴定出两个随机扩增多态性DNA （RAPD）标记与菜绿支原体抗性相关。第一个RAPD标记（B459(1600)）是一个1.6 kb的条带，在易感植物中存在，在抗性植物中不存在。第二个RAPD标记（B386(900)）是一个0.9 kb的条带，在抗性植株中存在，而在易感植株中不存在。每个RAPD标记的分离率为3:1，两个非连锁位点的分离率为9:3:3:1。在所研究的16个F-3家族中，有15个家族的标记物的基因型正确地预测了抗病基因型（杂合或纯合）。唯一的例外可能是B459（1600）和相关易感基因之间的重组。这些数据与假设一致，即在BAT-477中，对菜绿分枝杆菌的抗性由两个优势互补基因控制。这些基因被命名为Mp-1和Mp-2 （Macrophomina phaseolina）。
The inheritance of resistance to Macrophomina phaseolina was determined using traditional approaches and molecular markers. Inheritance studies were based on a cross between the resistant accession BAT-477 and the susceptible accession A-70. Resistance to M. phaseolina was examined by inoculating bean seeds with soil infested with sclerotia of M. phaseolina. The ratio of resistant to susceptible plants in the F-2 population was close to 9:7. Bulked segregant analysis identified two random amplified polymorphic DNA (RAPD) markers linked to M. phaseolina resistance. The first RAPD marker (B459(1600)) was a 1.6-kb band present in the susceptible and absent in the resistant plants. The second RAPD marker (B386(900)) was a 0.9-kb band present in the resistant, but absent in the susceptible plants. Each of the RAPD markers segregated in a 3:1 ratio as expected, and the segregation of both markers followed a 9:3:3:1 ratio expected for two unlinked loci. In 15 of the 16 F-3 families examined, the genotype of the markers correctly predicted the genotype (heterozygous or homozygous) for disease resistance. The one exception could be attributed to recombination between B459(1600) and the linked susceptible gene. These data were consistent with the hypothesis that, in BAT-477, resistance to M. phaseolina is controlled by two dominant complementary genes. The symbols Mp-1 and Mp-2 (Macrophomina phaseolina) were proposed for these genes.