Quantitative determination of carbaryl and carbaryl metabolites by reversed-phase high-performance liquid chromatography.
Quantitative determination of carbaryl and carbaryl metabolites by reversed-phase high-performance liquid chromatography.
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DOI：
10.1016/s0021-9673(01)94508-x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
S. Ward;G. May;R. Branch
中科院分区：
文献类型：
--
作者：
S. Ward;G. May;R. Branch
在10-ml容量的玻璃培养管中进行浸提。甲磺醇被用作内标物用于测定甲萘威和α-萘酚，而β-萘酚被用作羟基化代谢物的内标物。血浆或尿液样本（0.5至1.通过涡旋混合30秒，用5 ml己烷乙酸乙酯（3：1）萃取含有内标物（500 ng）的50 ml样品。离心（100 g，10 min）和分离后，在稳定的氮气流下将有机相蒸发至干。样品在乙腈（50 - 1）中复溶。将5-50 - 1的等分试样注射到柱上。
Extractions were carried out in lo-ml capacity glass culture tubes. Mesurol was used as the internal standard for the determination of carbaryl and cr-naphthol and betanol was used as the internal standard for the hydroxylated metabolites. Samples of plasma or urine (0.5 to 1. O ml) containing internal standard (500 ng) were extracted with 5 ml of hexaneethyl acetate (3: l) by vortex mixing for 30 s. After centrifugation (100 g for 10 min) and separation, the organic phase was evaporated to dryness under a steady stream of nitrogen. Samples were reconstituted in acetonitrile (50~ 1). An aliquot of 5-50~ 1 was injected onto the column.