Affinity chromatography of heme-binding proteins: an improved method for the synthesis of hemin-agarose.
Affinity chromatography of heme-binding proteins: an improved method for the synthesis of hemin-agarose.
复制标题
血红素结合蛋白的亲和层析：血红素琼脂糖合成的改进方法。
DOI：
10.1016/0003-2697(82)90475-4
复制
发表时间：
1982
影响因子：
2.9
通讯作者：
Mueller,GC
中科院分区：
文献类型：
--
作者：
Tsutsui,K;Mueller,GC
Hemin was conjugated to aminoethyl-agarose with high efficiency using 1,1′-carbonyldiimidazole as a condensing agent. The hemin-agarose was an efficient affinity resin for certain heme-binding proteins. It retained 4–5 mg of globin per milliliter of packed gel, whereas hemoglobin did not bind to the resin. The specificity of the adsorbent was further demonstrated by the selective binding of hemopexin from the whole sera of several species of a new heme-binding protein (HBP.93) from rabbit serum. Serum albumin, a major protein in serum which can also bind heme, did not bind to the adsorbent.
