Localized stabilization of microtubules by integrin- and FAK-facilitated Rho signaling
Localized stabilization of microtubules by integrin- and FAK-facilitated Rho signaling
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DOI：
10.1126/science.1091325
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发表时间：
2004-02-06
期刊：
影响因子：
56.9
通讯作者：
Gundersen, GG
中科院分区：
文献类型：
--
作者：
Palazzo, AF;Eng, CH;Gundersen, GG
微管（MT）稳定是由迁移细胞中的小鸟苷三磷酸（GTP）结合蛋白Rho及其效应物，哺乳动物同源物Diaphanous （mDia）调节的，但负责边缘局部稳定的因素尚不清楚。我们报道了整合素介导的局灶黏附激酶（FAK）在边缘的激活是小鼠成纤维细胞中rho -media信号通路稳定MT所必需的。MT稳定还涉及fak调节的脂质筏标记物神经节苷脂G（M1）定位到边缘。整合素- fak信号通路可能通过G（M1）或含有G（M1）的特殊膜结构域促进Rho-mDia信号传导，以稳定迁移细胞前沿的mt。
Microtubule (MT) stabilization is regulated by the small guanosine triphosphate (GTP)-binding protein Rho and its effector, mammalian homolog of Diaphanous (mDia), in migrating cells, but factors responsible for localized stabilization at the leading edge are unknown. We report that integrin-mediated activation of focal adhesion kinase (FAK) at the leading edge is required for MT stabilization by the Rho-mDia signaling pathway in mouse fibroblasts. MT stabilization also involved FAK-regulated localization of a lipid raft marker, ganglioside G(M1), to the leading edge. The integrin-FAK signaling pathway may facilitate Rho-mDia signaling through G(M1), or through a specialized membrane domain containing G(M1), to stabilize MTs in the leading edge of migrating cells.