Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice
Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice
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DOI：
10.1002/ptr.2163
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发表时间：
2007-09-01
影响因子：
7.2
通讯作者：
Gao, Dawei
中科院分区：
文献类型：
--
作者：
Li, Aan;Li, Qingwang;Gao, Dawei
龙葵 (SNL) 因其利尿和解热作用而在传统中药中使用了几个世纪。本研究检验了从龙葵 (SNL-P) 中分离的粗多糖对肿瘤生长的影响。 SNL-P对荷瘤小鼠宫颈癌（U14）有显着的生长抑制作用。进一步分析抑瘤机制发现，口服SNL-P 12 d后，宫颈癌切片中凋亡肿瘤细胞数量显着增加，Bax表达增加，Bcl-2和突变型p53表达显着降低。此外，SNL-P治疗降低了血清TNF-α水平。这些结果表明，SNL-P给药对肿瘤生长的抑制可能与血清TNF-α水平降低有关，导致肿瘤组织大量坏死，上调Bax，下调Bcl-2和突变型p53基因表达，从而引发肿瘤细胞凋亡。这些发现表明 SNL-P 是一种潜在的抗肿瘤剂。版权所有 (C) 2007 John Wiley & Sons, Ltd.
Solanum nigrum Linne (SNL) has been used in traditional Chinese medicine for centuries because of its diuretic and antipyretic effects. The present study examined the effect of the crude polysaccharides isolated from Solanum nigrum Linne (SNL-P) on tumor growth. SNL-P had a significant growth inhibition effect on cervical cancer (U14) of tumor-bearing mice. Further analysis of the tumor inhibition mechanism indicated that the number of apoptotic tumor cells increased significantly, the expression of Bax increased and the expression of Bcl-2 and mutant p53 decreased dramatically in cervical cancer sections after oral administration of SNL-P for 12 days. Moreover, SNL-P treatment decreased the level of blood serum TNF-alpha These results indicated that the tumor growth inhibition of SNL-P administration might correlate with the reduction of TNF-a level of blood serum, which resulted in a massive necrosis in tumor tissues and the up-regulation of Bax and down-regulation of Bcl-2 and mutant p53 gene expression, which triggered apoptosis in tumor cells. These findings demonstrated that the SNL-P is a potential antitumor agent. Copyright (C) 2007 John Wiley & Sons, Ltd.