Hitch-hiking from HRAS1 to the WAGR locus with CMGT markers
Hitch-hiking from HRAS1 to the WAGR locus with CMGT markers
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使用 CMGT 标记从 HRAS1 搭便车到 WAGR 基因座
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
D. Porteous
中科院分区：
文献类型：
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作者：
W. Bickmore;S. Christie;V. Heyningen;N. Hastie;D. Porteous
肾母细胞瘤与无虹膜、泌尿生殖系统异常和精神发育迟滞（WAGR综合征）的临床相关性的细胞遗传学特征是11号染色体短臂的可变长度、结构性缺失，其总是包括至少部分带11 p13。HRAS 1选择性染色体介导的基因转移（CMGT）产生了一个突变体，E65-6，其中唯一保留的人类基因映射到带11 p13或HRAS 1，在区域11p15.4-pter。从E65-6分离的人重组体被定位到一组五个WAGR缺失杂交体和两个临床相关的易位。我们发现E65-6对于11p15.4-pter标记富集一致400倍，对于11 p13标记富集一致200倍。从HRAS 1与CMGT标记的“搭便车”，使我们能够定义七个离散的间隔，对向带11 p13。这两个相关的易位共同位于WAGR基因座的最小重叠区域内，该区域已通过识别比FSHB更接近的新间隔而重新定义。
The clinical association of Wilms' tumour with aniridia, genitourinary abnormalities and mental retardation (WAGR syndrome) is characterised cytogenetically by variable length, constitutional deletion of the short arm of chromosome 11, which always includes at least part of band 11p13. HRAS1-selected chromosome mediated gene transfer (CMGT) generated a transformant, E65-6, in which the only human genes retained map either to band 11p13 or, with HRAS1, in the region 11p15.4-pter. Human recombinants isolated from E65-6 were mapped to a panel of five WAGR deletion hybrids and two clinically related translocations. We show that E65-6 is enriched congruent to 400-fold for 11p15.4-pter markers and congruent to 200-fold for 11p13 markers. 'Hitch-hiking' from HRAS1 with CMGT markers has allowed us to define seven discrete intervals which subtend band 11p13. Both associated translocations co-locate within the smallest region of overlap for the WAGR locus, which has been redefined by identifying a new interval closer than FSHB.