STUDIES ON PLANT-TISSUE CULTURE .59. BIOTRANSFORMATION OF 2-PHENYLPROPIONIC ACID IN ROOT CULTURE OF PANAX-GINSENG
STUDIES ON PLANT-TISSUE CULTURE .59. BIOTRANSFORMATION OF 2-PHENYLPROPIONIC ACID IN ROOT CULTURE OF PANAX-GINSENG
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DOI：
10.1016/0031-9422(89)80036-6
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发表时间：
1989-01-01
期刊：
影响因子：
3.8
通讯作者：
YOSHIKAWA, T
中科院分区：
文献类型：
--
作者：
FURUYA, T;USHIYAMA, M;YOSHIKAWA, T
人参的根培养物能够将（RS）-2-苯基丙酸转化为（RS）-2-苯基丙酰基β- d-吡喃葡萄糖苷，（2 RS）-2-O-（2-苯基丙酰基）-D-葡萄糖，（2S）-2-苯基丙酰基6-O-β- D-吡喃木糖基-β- D-吡喃葡萄糖苷和（R）-2-苯基丙酸的肌醇酯。樱草糖基酯和肌醇酯在C-2处由外消旋混合物立体选择性地形成。转化成葡糖基酯的转化率非常高，约为90%，在第三天总转化率达到100%。约有一半的产物分泌到培养基中。与根培养相比，愈伤组织的糖基化能力较低。
Root cultures of Panax ginsena are able to convert (RS)-2-phenylpropionic acid into (RS)-2-phenylpropionyl .beta.-d-glucopyranoside, (2RS)-2-O-(2-phenylpropionyl)-D-glucose, (2S)-2-phenylpropionyl 6-O-.beta.-D-xylopyranosyl-.beta.-D-glucopyranoside and a myo-inositol ester of (R)-2-phenylpropionic acid. The primeverosyl ester and myo-inositol ester were formed stereoselectively at C-2 from the racemic mixture. The conversion into glucosyl esters was very high at ca 90% and the total conversion reached 100% at day three. About a half of the products were escreted into the medium. Compared with the root culture, the callus showed lower glycosylation ability.