Semiquantitative distribution of galanin-receptor (GAL-R1) mRNA-containing cells in the male rat hypothalamus.
Semiquantitative distribution of galanin-receptor (GAL-R1) mRNA-containing cells in the male rat hypothalamus.
复制标题
雄性大鼠下丘脑中含有甘丙肽受体 (GAL-R1) mRNA 的细胞的半定量分布。
作者：
V. Mitchell;E. Habert;Jacques Epelbaum;Jean;J. Beauvillain
用35 S-核糖核酸探针原位杂交法研究了大鼠下丘脑甘丙肽受体（GAL-R1）mRNA的半定量分布。多数下丘脑核团表达GAL-R1 mRNA。在下丘脑前部，高水平的表达被发现在内侧视前区，室旁核和视上核。许多细胞也表达GAL-R1 mRNA，在室周区域中具有中等水平的表达。极少数GAL-R1表达细胞存在于视交叉上核。在下丘脑内侧核，在背内侧核和腹内侧核中检测到大量表达细胞。弓状核在其整个吻尾侧范围内中度标记;在腹内侧和腹外侧分区中也可见标记细胞体。这些结果表明，GAL-R1 mRNA不仅表达于下丘脑前核，而且还表达于下丘脑基底内侧核和室周区。这种下丘脑分布与125 I-GAL结合位点和GAL免疫反应纤维的分布密切相关。这种分布代表了GAL在神经内分泌、行为和自主神经功能的下丘脑调节中的作用的形态学基质。
The semiquantitative distribution of mRNA encoding for rat galanin receptor (GAL-R1) was examined by in situ hybridization in the rat hypothalamus using a 35S-riboprobe. Most hypothalamic nuclei expressed GAL-R1 mRNA. In the anterior hypothalamus, high levels of expression were found in the medial preoptic area, paraventricular and supraoptic nuclei. Numerous cells also expressed the GAL-R1 mRNA with a moderate level of expression in the periventricular region. Very few GAL-R1-expressing cells were present in the suprachiasmatic nucleus. In the medial hypothalamus, numerous expressing cells were detected in the dorsomedial and ventromedial nuclei. The arcuate nucleus was moderately labeled throughout its rostrocaudal extent; labeled cell bodies were visible in the ventromedial and ventrolateral subdivisions as well. These results indicate that the GAL-R1 mRNA is not only expressed in anterior hypothalamic nuclei but also in the mediobasal hypothalamus and periventricular region. This hypothalamic distribution correlates well with that of 125I-GAL-binding sites and GAL-immunoreactive fibers. This distribution represents the morphological substrate for GAL roles in the hypothalamic regulation of neuroendocrine, behavioral and autonomic functions.