Microfluidization as a potential technique to modify surface properties of soy protein isolate
Microfluidization as a potential technique to modify surface properties of soy protein isolate
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微流化作为改变大豆分离蛋白表面特性的潜在技术
DOI：
10.1016/j.foodres.2012.03.006
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发表时间：
2012-08-01
影响因子：
8.1
通讯作者：
Tang, Chuan-He
中科院分区：
文献类型：
--
作者：
Shen, Lan;Tang, Chuan-He
研究了在特定压力水平（120 MPa）下微射流处理对未加热和预热的大豆分离蛋白（SPI）的理化、构象和乳化特性的影响。热预处理分别在75、85和95℃的温度下进行。所测试的特性包括蛋白质溶解度（PS）、表面疏水性（H - o）、游离巯基（SH）和二硫键（SS）含量、构象特征以及乳化特性（例如乳化能力指数（EAI）、乳液中油滴的粒径、乳析指数）。结果表明，微射流处理通常以依赖于预热温度的方式提高了未加热和预热SPI的PS、H - o、SS含量以及EAI；该处理还导致蛋白质部分展开和变性，甚至结构重排；该处理提高了乳化效率和抗乳析稳定性，但加速了预热SPI稳定乳液中油滴的桥连絮凝，在预处理温度较高时观察到的絮凝程度更高。尺寸排阻色谱和SDS - PAGE分析证实，微射流处理导致不溶性聚集体转化为可溶性聚集体，其中可能涉及SH/SS互换。研究结果表明，微射流处理，或者与热预处理相结合，将是一种改善SPI表面特性的有效技术。（C）2012爱思唯尔有限公司。保留所有权利。
Modifications in physicochemical, conformational and emulsifying properties of unheated and preheated soy protein isolates (SPIs) by microfluidization at a specific pressure level (120 MPa) were investigated. The heat pretreatment was carried out at temperatures of 75, 85 and 95 degrees C, respectively. The tested properties included protein solubility (PS), surface hydrophobicity (H-o), free sulfhydryl group (SH) and disulfide bond (SS) contents, conformational characteristics, as well as emulsifying properties (e.g. emulsifying ability index (EAI), particle size of oil droplets in the emulsions, creaming index). The results indicated that the microfluidization treatment generally increased PS, H-o, SS content, as well as EAI of both unheated and preheated SPIs, in a preheating temperature dependent way; the treatment also resulted in partial unfolding and denaturation, and even structural re-arrangement of the proteins; the treatment improved the emulsifying efficiency and stability against creaming, but accelerated the bridging flocculation of oil droplets in the preheated-SPI-stabilized emulsions, with higher extent of flocculation observed at higher temperatures of the pretreatment. Size exclusion chromatography and SDS-PAGE analyses confirmed that the microfluidization led to transformation of insoluble aggregates into soluble ones, in which SH/SS interchanges might be involved. The findings suggest that the microfluidization treatment, or in combination with a thermal pretreatment, would be an effective technique to improve surface properties of SPI. (C) 2012 Elsevier Ltd. All rights reserved.